Arch Plast Surg.  2012 Sep;39(5):452-456. 10.5999/aps.2012.39.5.452.

The Effect of Phosphatidylcholine and Deoxycholate Compound Injections to the Localized Adipose Tissue: An Experimental Study with a Murine Model

• Noh Y
• Heo CY

• KMID: 2267706
• DOI: http://doi.org/10.5999/aps.2012.39.5.452

Abstract

BACKGROUND
Phosphatidylcholine (PPC) and deoxycholate (DCA) compound has been recently used for the purpose of partial lipolysis and is valued for its efficacy and lower invasiveness compared to liposuction and dermolipectomy used previously. In this article, the authors discuss the efficacy of the PPC dissolved in DCA via an experimental rat study model, along with suggesting a useful animal experimental model for the study of adipose tissue and lipolysis.
METHODS
Bilateral inguinal fat pads of an experimental rat were elevated with the deep inferior epigastric vessel as the sole vascular pedicle. Normal saline was injected on one side as a control group and a PPC and DCA compound was injected on the other side. After 4 days, the rats were euthanized for microscopic tissue examination. The pathology was scored by a semiquantitative system in 4 categories: normal fat amount, fat necrosis, inflammatory activity, and stage of fibrosis. A Wilcoxon signed-rank test powered by SPSS packet program was used for statistical analysis and to determine significance.
RESULTS
Microscopic examination was performed on the obtained samples, and the experimental data of all four categories showed significant histologic differences compared to the control group. All of the data also showed statistical significance by the Wilcoxon signedrank test (P<0.01).
CONCLUSIONS
In the inguinal fat pad rat model, the control group and the experimental group had a differed significantly in the amount of normal fat tissue, inflammation, necrosis, and fibrosis. We recommend the rat inguinal fat pad model used in this study, as it is likely to be useful in related research.

Keyword

Phosphatidylcholine; Lipolysis; Fat body; Deoxycholate

MeSH Terms

Adipose Tissue
Animal Experimentation
Animals
Deoxycholic Acid
Fat Body
Fat Necrosis
Fibrosis
Glycosaminoglycans
Inflammation
Lipectomy
Lipolysis
Necrosis
Phosphatidylcholines
Rats
Deoxycholic Acid
Glycosaminoglycans
Phosphatidylcholines