Korean J Nutr.  2009 Apr;42(3):207-212. 10.4163/kjn.2009.42.3.207.

Effects of Plant Water Extract Codonopsis Lanceolatae on Mouse Immune Cell Activation Ex Vivo

• Ryu HS
• Kim KO
• Kim HS

• KMID: 2264720
• DOI: http://doi.org/10.4163/kjn.2009.42.3.207

Abstract

Codonopsis lanceolatae has been used as one of the traditional remedies as well as food source. However, few studies on their immunomodulating effects have been reported. We previously reported that ex vivo supplementation of Codonopsis lanceolatae water extracts enhanced splenocyte proliferation compared to the control group. In order to elucidate its ex vivo effect, six to seven week old balb/c mice were fed ad libitum on a chow diet and water extracts of Codonopsis lanceolatae were orally administrated every other day for four weeks at two different concentrations (50 and 500 mg/kg B.W.). After preparing the single cell suspension, the proliferation of splenocytes was determined by MTT (3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. The production of cytokine (IL-1beta, IL-6, TNF-alpha), secreted by macrophages stimulated with LPS or not, was detected by ELISA assay using a cytokine kit. After 48 hrs of incubation with the mitogen (ConA or LPS) stimulation, the mice splenocyte proliferation in experimental group was statistically increased at two different concentrations than that in control group. The cytokines production was more significantly enhanced at the lower supplementation (500 mg/kg B.W.) group rather than higher concentration (500 mg/kg B.W.) compared to the control group. The results of this study may suggest that the supplementation of water extract of plant mixture could regulate the immune function by increasing the splenocyte proliferation and enhance the immune function through regulating cytokine production capacity by activated macrophages in mice.

Keyword

splenocyte proliferation; IL-1beta; IL-6; TNF-alpha; cytokine

MeSH Terms

Animals
Codonopsis
Cytokines
Diet
Enzyme-Linked Immunosorbent Assay
Interleukin-6
Macrophages
Mice
Plants
Tumor Necrosis Factor-alpha
Water
Cytokines
Interleukin-6
Tumor Necrosis Factor-alpha
Water

Figure

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  Fig. 1 Flow diagram for water extraction procedure.

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  Fig. 2 Study design of ex vivo experiment.

Reference

1. Hong WS, Lee JS, Ko SY, Choi YS. A study on the perception of Codonopsis lanceolatae dishes and the development of Codonopsis lanceolatae dishes. Korean J Food Cookery Sci. 2006. 22(2):181–192.

2. Lim SD, Seong KS, Kim KS, Han DU. Effect of fermented milk containing herb extract from acnthopanax lanceolata on the immune status of mouse. Korean J Food Sci Ani Resour. 2007. 27(1):95–101.

3. Jang JH. Sanyacho dongeubogam. 2001. Seoul: Academy Press;156–157.

4. Lee SK. The volatile flavor components of wild & cultivated codonopsis lanceolata by instrumental and sensory analysis [Master thesis]. 1999. Seoul: Duksung Woman's Univ..

5. Kim EJ, Ahn MS. Antioxidative effect of ginger extracts. Korean J Food Sci. 1993. 9(1):37–42.

6. Hwang EJ, Cha YU, Park MH, Lee JW, Lee SY. Cytotoxicity and chemosensitizing effect of camelia tea extract. J Korean Soc Food Sci Nutr. 2004. 33:487–493.

7. Arai S. Studies on function foods in Japan-state of Art. Biosci Biotechnol Biochem. 1996. 60:9–15.

8. Kang DH, Lee SK, Kyng HR. Separation of phospholipids from soybean by NP-HPLC with ELSD. Kor J Chem Eng. 2002. 19(5):818–820.

9. Cheng GC, Lee JY, Kim DC, Suh SO, Hwang WI. Inhibitory effects of salvia miltiorrhiza extract on growth of some cancer cells. J Korean Soc Food Sci Nutr. 2000. 29:726–731.

10. Ji WD, Jeong HC, Lee SJ, Chun YG. Antimicrobial activity and distilled components of garlic and ginger. J Agric Chem Biotechnol. 1997. 40:514–518.

11. Park JK, Kim YH, Kim KS, Kwang J. Volatile flavor components of Codonopsis lanceolatae traut. J Korean Agric Chem Soc. 1989. 32(3):338–343.

12. Maeng YS, Park HK. Antioxidant activity of ethanol extract from Dodok. Korean J Food Sci Technol. 1991. 23(3):311–318.

13. Han EG, Sung IS, Moon HG, Cho SY. Effect of Codonopsis lanceolatae water extract on the level of lipid in rats fed high fat diet. J Korean Soc Food Sci Nutr. 1998. 27(1):940–944.

14. Sun JS. Effect of Codonopsis lanceolatae radix water extract on immunocytes. Korean J Food & Nutr. 1996. 9(4):379–384.

15. Suh JS, Eun JS. Isolation of active components on immunocytes from codonopsis lanceolatae. Korean J Community Nutrition. 1998. 31(6):1076–1081.

16. Ryu HS, Kim HS. Effects of Job's Tear extracts on mouse immune cell activation. J Korean Dietetic Assoc. 2005. 11(1):44–50.

17. Park KY, Lee SJ, Lee KI, Rhee SH. The antitumor effect in sarcoma-180 tumor cell of mice of mice administered with japanese apricot, garlic ginger doenjang. Korean J Food Cookery Sci. 2005. 21(5):599–606.

18. Jung YH. Effect of Hizikia fusiforme water extracts on mouse immune cell activation [dissertation]. 2003. Seoul: Sookmyung Women's Univ..

19. Ryu HS, Kim HS. Effect of sorghum bicolor L. moench (sorghum, su-su) water extracts on mouse immune cell activation. Korean J Food & Nutr. 2006. 19(2):176–182.

20. Mishell BB, Shiigi SM. Selected methods in cellular immunology. 1980. 1st ed. Sanfrancisco: WHFreemanandco;4–27.

21. Ryu HS, Kim HS. Enhancing Effects of Ginger (zingiber officinale Roscoe) Extracts on Mouse Spleen and Macrophage Cells Activation. Korean J Nutr. 2004. 37(9):780–785.

22. Ryu HS, Jung YH, Kim HS. Effect of Hizikia fusiforme water extracts on mouse immune cell activation. Korean J Nutr. 2007. 40(7):639–649.

23. Kim SH. Effect of Phlomis umbrosa Turcz ethanol extract on mouse splenocyte proleferation and cytokine production by activated peritoneal [Mater thesis]. 2001. Seoul: Sookmyung Women's Univ..

24. Lim SD, Kim KS, Do JR. Effect of Codonopsis lanceolatae on macrophage activity and on the growth of lactic starter during fermentation. Korean J Food Sci Ani Resour. 2006. 26(1):136–143.

25. Ryu HS, Kim HS. Effect of sorghum bicolor L. moench (sorghum, su-su) water extracts on mouse immune cell activation. Korean J Food & Nutr. 2006. 19(2):176–182.

26. Park HA. Enhancing effect of Ixeris sonchifolia hance Oenanthe javanica, and Fagopyrum escluentum Moench on mouse immune cell activation [Mater thesis]. 2003. Seoul: Sookmyung Women's Univ..

27. Kim SH. Effect of phlomis umbrosa turcz ethanol extract on mouse splenocyte proleferation and cytokine production by activated peritoneal [Mater thesis]. 2003. Seoul: Sookmyung Women's Univ..

28. Ramunans Z, Robert HB, Max L, Paricia JM. In vivo effect of chronic treatment with (met5)-enkephalin on hematological values and natural killer cell activity in athvity in athymic mice. Life Sci. 2000. 66(9):829–834.