Korean J Nutr.  2008 Mar;41(2):127-134.

The Role of The Effects of Vitamin D Supplementation on Bone Mineral Density and Bone Mineral Content in Ovariectomized Rats Compensation in Rats

• Choi MJ
• Kim SM

Abstract

The effects of vitamin D3 supplementation on bone mineral density and bone mineral content in ovariectmized rats were investigated. Forty female Sprague-Dawley rats (body weight 180 g) were divided into two group, ovariectomy and Sham groups, which were each randomly divided into two subgroups that were fed casein and casein supplemented with vitamin D3 diets. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin, USA) in spine and femur on 6 weeks after feeding. Osteocalcin and urinary DPD crosslinks value were measured as markers of bone formation and resorption. The body weight gain and food efficiency ratio (FER) were higher in OVX groups than in Sham groups regardless of diets. Serum Ca concentration and urinary Ca excretion were higher in vitamin D3 supplemented group than in casein group in Sham and OVX. Crosslinks values were higher in OVX groups than in Sham groups. Spine BMD and femur BMD of ovariectomy group were significantly lower than Sham groups, however vitamin D3 supplemented groups were significantly higher than control groups in Sham and OVX. In conclusion, dietary vitamin D3 supplementation on ovariectimized rats were significantly increased bone mineral density and bone mineral content in spine and femur.

Keyword

Vitamin D3; Bone mineral density; OVX

MeSH Terms

Animals
Body Weight
Bone Density
Caseins
Cholecalciferol
Compensation and Redress
Diet
Female
Femur
Humans
Osteocalcin
Osteogenesis
Ovariectomy
Rats
Rats, Sprague-Dawley
Salicylamides
Spine
Vitamin D
Vitamins
Wisconsin
Caseins
Cholecalciferol
Osteocalcin
Salicylamides
Vitamin D
Vitamins