Korean J Obstet Gynecol.  1999 Jan;42(1):98-103.

Correlation between Apoptosis and Proliferative Activity in Squamous Cell Neoplasia of Uterine Cervix

• Moon YH
• Lee SK

Abstract

OBJECTIVE
Quantitative analysis of both apoptosis and proliferation should provide important insight into tumor biology. The aim of this study is to clarify the significance of apoptosis in the progression of uterine cervical neoplasia, including cervical intraepithelial neoplasia, and microinvasive and invasive squamous cell carcinoma categories. in relation to cell proliferation.
METHODS
: Forty five cases of cervical intraepithelial neopasia. 10 of microinvasive carcinoma, and 10 of invasive squamous cell carcinoma were examined using formalin fixed and paraffin embedded samples. The TdT mediated dUTP-biotin nick end labelling(TUNEL) method for apoptotic cells, and Ki-67 and Topo-II immunojhistochemistry for proliferating activity were performed.
RESULTS
Apoptotic and proliferative indices, calculated by counting positive nuclei among 1,000 nuclei of cancer cells, should significant positive correlation with histological malignancy grading in CIN and tumor cell invasion into stroma, and showed also significant positive correlation between them.
CONCLUSION
These findings suggest that apoptosis in cervical neoplasia may be closely related to tumor cell differentiation and progression.

Keyword

Apoptosis; CIN of uterine cervix; TUNEL method; Ki-67; Topo-II immunohistochemistry

MeSH Terms

Apoptosis*
Biology
Carcinoma, Squamous Cell
Cell Differentiation
Cell Proliferation
Cervical Intraepithelial Neoplasia
Cervix Uteri*
Female
Formaldehyde
Paraffin
Formaldehyde
Paraffin