Allergy Asthma Immunol Res.  2010 Apr;2(2):134-140. 10.4168/aair.2010.2.2.134.

IL-13 Gene Polymorphisms are Associated With Rhinosinusitis and Eosinophilic Inflammation in Aspirin Intolerant Asthma

• Palikhe NS
• Kim SH
• Cho BY
• Choi GS
• Kim JH
• Ye YM
• Park HS

• KMID: 2260600
• DOI: http://doi.org/10.4168/aair.2010.2.2.134

Abstract

PURPOSE
Aspirin-intolerant asthma (AIA) is characterized by moderate to severe asthma that is aggravated by aspirin or other non-steroidal anti-inflammatory drugs. Affected patients frequently have chronic rhinosinusitis and nasal polyposis due to persistent upper and lower airway inflammation with marked eosinophilia. IL-13 plays a crucial role in the development of allergic asthma by inducing airway eosinophilia and hyper-reactivity and it has been correlated with an increased eosinophil count.
METHODS
Two promoter polymorphisms of the IL-13 gene (-1510 A>C and -1055C>T) and one coding nonsynonymus Arg110Gln (110G>A) polymorphism were genotyped using primer extension methods in 162 patients with AIA, 301 patients with aspirin-tolerant asthma (ATA), and 430 normal healthy controls (NC).
RESULTS
There was no significant difference in the genotype, allele, and haplotype frequencies of the three polymorphisms among the three groups. AIA patients with the AA genotype -1510A>C (P=0.012) and CC genotype -1055C>T (P<0.001) had a significantly higher frequency of rhinosinusitis, as compared to those with the minor alleles of these two single nucleotide polymorphisms. AIA patients with the GG genotype had a higher peripheral eosinophil count (P=0.025) and a higher serum eotaxin-1 level (P=0.044), as compared to patients with the AA genotype IL-13 Arg110Gln (110G>A).
CONCLUSIONS
These findings suggest that the IL-13 polymorphisms at -1510A>C and 1055C>T are associated with the development of rhinosinusitis in AIA patients. IL-13 Arg110Gln may be associated with an increased eosinophil count and eotaxin-1 level and could increase eosinophilic inflammation in the upper and lower airways of patients with AIA.

Keyword

IL-13; polymorphisms; aspirin intolerant asthma; eosinophilic inflammation; rhinosinusitis

MeSH Terms

Alleles
Aspirin
Asthma
Chemokine CCL11
Clinical Coding
Eosinophilia
Eosinophils
Genotype
Haplotypes
Humans
Inflammation
Interleukin-13
Polymorphism, Single Nucleotide
Aspirin
Chemokine CCL11
Interleukin-13

Figure

• 

  Fig. 1 A: The peripheral log total eosinophilic count (TEC) according to IL-13 Arg110Gln (110G>A) polymorphism. B: The serum eotaxin-1 level according to IL-13 Arg110Gln (110G>A) gene polymorphism in AIA patients. C: The serum eotaxin-1 level according to IL-13 Arg110Gln (110G>A) gene polymorphism in ATA patients.

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