Asia Pac Allergy.  2019 Jul;9(3):e22. 10.5415/apallergy.2019.9.e22.

Association of IL-1 gene polymorphisms with chronic rhinosinusitis with and without nasal polyp

• Mohamad S
• Hamid SS
• Azlina A
• Md Shukri

• KMID: 2454156
• DOI: http://doi.org/10.5415/apallergy.2019.9.e22

Abstract

BACKGROUND
Chronic rhinosinusitis (CRS) is one of the most common and complex chronic inflammatory disease of sinonasal mucosa. Even though the pathogenesis of CRS is multifactorial and still unclear, the role of cytokines especially interleukin-1 (IL-1) is being investigated worldwide in different population because of varying results obtained.
OBJECTIVE
To study the association of IL-1 (A and B) gene polymorphisms with chronic rhinosinusitis with nasal polyp (CRSwNP) and without nasal polyp (CRSsNP), and other factors related.
METHODS
This is a case-controlled study which include a total of 138 subjects recruited from Otorhinolaryngology-Head and Neck Surgery clinic in Hospital Universiti Sains Malaysia. Genotyping of the IL-1A (+4845G, +4845T) and IL-1B (−511C, −511T) were performed with restriction fragment length polymorphism analysis.
RESULTS
There was a statistical significant association between IL-1B (−511C, −511T) polymorphism with CRSwNP and CRSsNP (p < 0.001). The CT genotype of IL-1B was markedly increased in CRSwNP subjects (52.2%). However, there was no significant association found between IL-1A (+4845G, +4845T) with CRSwNP and CRSsNP (p = 0.093). No association was found in factors related to CRS, which included asthma, atopy, allergy, aspirin sensitivity, and family history of nasal polyp (p value of 0.382, 0.382, 0.144, >0.95, and 0.254, respectively).
CONCLUSION
This study indicates an association of IL-1B (−511C, −511T) polymorphism with CRSwNP and CRSsNP in our population, hence there is a possibility of IL-1B involvement in modulating pathogenesis of CRS. There was no significant association of IL-1A (+4845G, +4845T) polymorphism with CRSwNP and CRSsNP, and other factors related.

Keyword

Rhinosinusitis; Nasal polyps; Interleukin-1; Single nucleotide polymorphism

MeSH Terms

Aspirin
Asthma
Case-Control Studies
Cytokines
Genotype
Humans
Hypersensitivity
Interleukin-1*
Malaysia
Mucous Membrane
Nasal Polyps*
Neck
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Aspirin
Cytokines
Interleukin-1

Figure

• 

  Fig. 1. A restriction fragment length polymorphism analysis of IL-1A (+4845G, +4845T) gene. Lane 1, homozygous wild (GG); lanes 4 and 5, heterozygous mutant (GT); lanes 2 and 3, homozygous mutant (TT); M, 50-bp DNA ladder. bp, base pair.

• 

  Fig. 2. A restriction fragment length polymorphism analysis of IL-1B (−511C, −511T) gene. Lanes 2 and 4, homozygous wild (CC); lanes 1 and 3, heterozygous mutant (CT); lanes 5 and 6, homozygous mutant (TT); M, 50-bp DNA ladder. bp, base pair.

Reference

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