Korean J Nephrol.  2008 Jul;27(4):465-475.

Association of Transforming Growth Factor-beta1 and Vascular Endothelial Growth Factor Gene Polymorphisms with Chronic Allograft Nephropathy and Graft Survival in Korean Renal Transplant Recipients

• Cho JH
• Ryu HM
• Jin MK
• Chun JH
• Hyun SH
• Choi JY
• Hur IK
• Lee EY
• Park SH
• Kim YL
• Kim CD

Abstract

PURPOSE: Transforming growth factor-beta1 (TGF-beta1) has been associated with the promotion of renal allograft interstitial fibrosis and thereby chronic allograft nephropathy (CAN). Vascular endothelial growth factor (VEGF) has been shown to contribute to cytoprotection of the graft after kidney transplantation. We investigated the influence of single nucleotide polymorphisms (SNPs) of the TGF-beta1 (C-509T and T869C) and the VEGF gene (C-2578A and C405G) on graft survival and the development of CAN.
METHODS
Genotyping was carried out using a real-time polymerase chain reaction which was performed on the LightCycler480 in 221 Korean renal transplant recipients and 148 healthy controls. According to the presence of CAN or chronic calcineurin inhibitor nephrotoxicity, the recipients were separated into the CAN (n=21) and the No CAN (n=200) groups.
RESULTS
The genotype frequencies of the SNPs were in Hardy-Weinberg equilibrium. The distributions of genotypes and alleles did not differ between recipients and controls. No significant differences were observed in the genotype distributions and allele frequencies between the CAN and the No CAN groups. The frequencies of haplotypes were not significantly different between the two groups, either. There were no statistically significant effects of TGF-beta1 and VEGF gene polymorphisms on graft survival.
CONCLUSION
This study did not show any statistically significant effects of four selected SNPs of the TGF-beta1 and the VEGF genes on the development of CAN and graft survival in Korean renal transplant recipients.

Keyword

Transforming growth factor-beta1; Vascular endothelial growth factor; Single nucleotide polymorphism; Kidney transplantation; Graft survival

MeSH Terms

Alleles
Calcineurin
Cytoprotection
Fibrosis
Gene Frequency
Genotype
Graft Survival
Haplotypes
Kidney Transplantation
Polymorphism, Single Nucleotide
Real-Time Polymerase Chain Reaction
Transforming Growth Factor beta1
Transplantation, Homologous
Transplants
Vascular Endothelial Growth Factor A
Calcineurin
Transforming Growth Factor beta1
Vascular Endothelial Growth Factor A