Korean J Gynecol Oncol Colposc.  1990 Mar;1(1):80-89.

DNA Patterns by Flow Cytometric Analysis of Squamous Carcinoma Cells of the Cervix Uteri : Relations with Prognosis and Biochemical Characteristics

• Nha DJ
• Kim SP

Abstract

This study was performed to find out the relationship between DNA pattern by flow cytometry and the prognosis of cervical squamous cell carcinoma, The subjects were 71 patients who were admitted to Catholic University Medical College Kangnam St. Marys Hospital from September 1983 to May 1989 under the diagnosis of cervical squamous cell carainoma. Samples were obtained from the subjects before chemotherapy or radiation therapy, and a through follow up stduy was carried out an the subjects. The authors obserued the relations between flow cytometric DNA pattern and clinical stage, age of patients, histopathological findings and tumor markers, with the following results. 1. The S-phase of the cell cycle increased significantly in Stages I, ll and III-IV compared to that of Stage O.However, there was no difference between Stages in G0/G1 phase and G2/M phase. 2. The incidence of aneuploid tumor increased significantly according to stage with the rates of increase reaching 22, 22%, 31.57%, 44.44% and 68.75% respectively in Stages 0, I, II, and Ill-lV. 3. The incidence of aneuploid tumor increased significantly depending on the age of patients with the rates standing at 21.42%, 31.58%, 40.90% and 68.75% respectively in the groups of below 40, 41-50, 51-60, and 61 years and over. 4. As for the relation between histopathological findings and the incidenee of aneuploid tumor, no difference was found according to the degree of differentiation, but there were significant differences between the keratinization group(23.07%) and the non keratinization group(51.11%).

MeSH Terms

Aneuploidy
Carcinoma, Squamous Cell*
Cell Cycle
Cervix Uteri*
Diagnosis
DNA*
Drug Therapy
Female
Flow Cytometry
Follow-Up Studies
Humans
Incidence
Prognosis*
Biomarkers, Tumor
DNA