Comparative study between cytogenetic analysis and flow cytometric analysis in transitional cell carcinoma of the urinary bladder

Jeong, Kang Seop; Yoon, Duck Ki

Abstract

Both cytogenetic analysis and flow cytometric analysis (FCM) are well known prognostic indicators in the transitional cell carcinoma (TCC) of the urinary bladder. To correlate results of the two methods, FCM cell DNA studies and cytogenetic analysis were performed concurrently on clinical samples from the twelve patients with TCC of urinary bladder and the cells taken from these comparisons were possible showed concordance between FCM and cytogenetics with respect to the presence or absence of aneuploidy. Among the four cases with discrepancies, three (27.2 % of all cases) showed peridiploid pattern by cytogenetic analysis and had tetraploid aneuploid DNA histograms. In one case with hyperdiploid pattern by cytogenetics (9.1 %) showed diploid on the nine patients with peridiploid pattern one has recurred. Also, two of the three patients with aneuploid pattern by cytogenetic study were recurred. In flow cytometric analysis, all of the nine patients with diploid pattern were not recurred. Whereas, of the nine cases of aneuploid patterns five were recurred. Although the number of samples were too small to compare the correlations statistically, our results indicate that these two methods are not related to each other in predicting the prognosis in transitional cell carcinoma of the urinary bladder.