Korean J Lab Med.
2002 Dec;22(6):447-451.
Evaluation of Nested Multiplex PCR in the Diagnosis of Malaria Infection
- Affiliations
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- 1Department of Laboratory Medicine, College of Medicine, Korea University, Korea. malarim@korea.ac.kr
- 2Neodin Incorporated, Seoul, Korea.
Abstract
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BACKGROUND: A peripheral blood smear has been the gold standard method for the diagnosis of malaria infection. Recently, many other methods have been introduced, although having inferior sensitivity and specificity to peripheral blood smears. We evaluated Neodin malaria PCR kit and its applicability in clinical settings.
METHODS
Samples from seventy patients who visited Korea University hospital were used for evaluation. DNA from EDTA blood was tested in nested multiplex PCR and 470 bp for Plasmodium vivax or 340 bp for Plasmodium falciparum was confirmed after electrophoresis. The detection limit was determined by dilution of malaria positive blood with normal blood.
RESULTS
Thirty-five cases of P. vivax and 10 cases of P. falciparum were noted. Except for a case of falciparum malaria, all positive cases were consistent with the peripheral blood smear results. Detection limit was 3.6 parasite/microL.
CONCLUSIONS
Neodin malaria nested multiplex PCR has high sensitivity and the ability for species discrimination and may be available in the diagnosis of malaria infection.
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