Korean J Lab Med.  2006 Apr;26(2):71-76. 10.3343/kjlm.2006.26.2.71.

Association of NQO1 Polymorphism with Multiple Myeloma Risk in Koreans

• Kang SH
• Kim TY
• Kim HY
• Lee YK
• Moon HW
• Lee DS
• Cho HI

• KMID: 2238893
• DOI: http://doi.org/10.3343/kjlm.2006.26.2.71

Abstract

BACKGROUND: NAD(P)H:quinone oxidoreductase 1 (NQO1) is an important enzyme in the metabolism of xenobiotics. NQO1 609C -> T (NQO1*2) has been reported to be associated with reduced enzyme activity, benzene-induced hematotoxicity, and acute leukemia. Incidences of multiple myeloma show interethnic variation and exposure to asbestos, petroleum products, and diesel exhaust is a risk factor for multiple myeloma. We studied the associations of NQO1 polymorphism with multiple myeloma risk, stage, and prognostic factors (hemoglobin, calcium, beta2-microglobulin, M-protein and creatinine).
METHODS
The frequency of NQO1 polymorphism was investigated in 117 myeloma patients and 166 controls. NQO1 genetic polymorphism was determined by TaqMan allelic discrimination assay. Prognostic factors were obtained from the patients' medical records.
RESULTS
The frequencies of the NQO1*1/*1, *1/*2, and *2/*2 genotypes were 31.6%, 63.2%, and 5.1% in the patients, whereas the respective figures in the controls were 31.9%, 48.3%, and 19,9%. The frequency of NQO1*2/*2 was significantly lower in patients than in controls and the odds ratio (OR) was 0.24 (95% confidence interval: 0.01-0.68) to NQO1*1/*1 genotype, indicating a decreased risk for multiple myeloma. There were no significant differences in tumor stages and other prognostic factors among NQO1 genotypes.
CONCLUSIONS
A risk for multiple myeloma decreased in NQO1*2/*2 genotype in Koreans. We report, for the first time, that NQO1 polymorphism is associated with multiple myeloma risk.

Keyword

NAD(P)H:quinone oxidoreductase 1; Multiple myeloma; Single nucleotide polymorphism

MeSH Terms

Asbestos
Calcium
Discrimination (Psychology)
Genotype
Humans
Incidence
Leukemia
Medical Records
Metabolism
Multiple Myeloma*
Odds Ratio
Petroleum
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Risk Factors
Vehicle Emissions
Xenobiotics
Asbestos
Calcium
Petroleum
Vehicle Emissions
Xenobiotics

Figure

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  Fig. 1. Detection of NAD(P)H:quinone oxidoreductase 1 (NQO1) polymorphism using TaqMan allelic discrimination. NQO1 genotypes were selected based on the ratio of the FAM and VIC intensity. A, NQO1*1/*1; B, NQO1*1/*2; C, NQO1*2/*2; D, no template control.

Reference

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