Korean Diabetes J.  2009 Feb;33(1):24-30. 10.4093/kdj.2009.33.1.24.

An Association between 609 C --> T Polymorphism in NAD(P)H: Quinone Oxidoreductase 1 (NQO1) Gene and Blood Glucose Levels in Korean Population

Affiliations
  • 1Department of Internal Medicine, Dankook University College of Medicine, Cheonan, Korea. dh9070@hanmail.net

Abstract

BACKGROUND: NAD(P)H: quinone oxidoreductase 1 (NQO1), which is an obligate two-electron reductase that utilizes NAD(P)H as an electron donor and is involved in the protection against oxidative stress, is likely involved in beta-cell destruction. We evaluated the frequency of the NQO1 polymorphism and its association with blood glucose levels.
METHODS
Genotypes were determined using a polymerase chain reaction restriction fragment length polymorphism-based assay in 56 patients and 48 healthy subjects. Fasting blood glucose, insulin, and lipid profiles were measured and homeostasis model assessment (HOMA)-insulin resistance (IR) was calculated from fasting glucose and insulin levels in the healthy subjects.
RESULTS
The genotype frequencies of NQO1 polymorphism were C/C (56.7%), C/T (42.3%), and T/T (1.0%). There were no associations between the NQO1 polymorphism and body mass index, blood pressure, lipid profile, HbA1c, postprandial glucose, and HOMA-IR. However, NQO1 mutants (C/T and T/T) showed weak but significantly higher fasting blood glucose levels compared with wild type (C/C).
CONCLUSION
Our data suggest that NQO1 609 C --> T polymorphism may be associated with glucose metabolism.

Keyword

Blood glucose; Human NQO1; Single nucleotide polymorphism

MeSH Terms

Benzoquinones
Blood Glucose
Blood Pressure
Body Mass Index
Electrons
Fasting
Genotype
Glucose
Homeostasis
Humans
Insulin
Oxidative Stress
Oxidoreductases
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Tissue Donors
Benzoquinones
Blood Glucose
Glucose
Insulin
Oxidoreductases

Figure

  • Fig. 1 The distributions of genotypes in subjects. A. Total. B. Men. C. Women.


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