Korean J Anesthesiol.  2007 Feb;52(2):194-201. 10.4097/kjae.2007.52.2.194.

Effect of Methylmethacrylate Monomer in Isolated Rat Tracheal Rings

• Gill HJ
• Lee JU
• Kim YH
• Yang HJ

• KMID: 2236623
• DOI: http://doi.org/10.4097/kjae.2007.52.2.194

Abstract

BACKGROUND
Methylmethacrylate monomer (MN) bone cement is commonly employed in orthopedic procedures, particularly total hip and knee replacement, to anchor prosthetic devices to bone. Numerous cardiopulmonary complications can occur just after injection of MN. And MN produces direct relaxation of vascular smooth muscle in vitro. The purpose of this study was to determine if MN could have relaxation effect in tracheal smooth muscle too.
METHODS
Each ring of rat trachea was suspended on wire supports in a bath with Tris Tyrode solution. Dose response curves of MN were recorded after contraction of tracheal ring with acethylcholine (Ach) 10(-5) M or cabachol (Cch) 10(-8) M. MN was administered in denuded tracheal rings and compared it's effect with intact tracheal rings to see the effect of epithelium for contraction. And MN dose response curves were recorded after pretreatment of nitric oxide synthase inactivator (L-NAME), muscarinic receptor blocker (atropine), beta-adrenaline receptor blocker (propranolol), adenylyl cyclase inhibitor (SQ22536) respectively. The effects of MN on cellular Ca2+ and K+ migration in rat tracheal preparations were studied.
RESULTS
MN significantly inhibited acetylcholine or carbachol induced contractions of tracheal rings dose-dependently (P < 0.05). This relaxation effect of MN was not recovered in denuded tracheal rings. And pretreatment with L-NAME, propranolol, atropine, SQ22536 or tetraethylammonium respectively did not recover the relaxation effect of MN. MN inhibited both intracellular calcium release and extracelluar calcium influx.
CONCLUSIONS
The relaxation effects of MN on rat tracheal rings are not related with epithelium, nitric oxide, muscarinic, or beta-adrenergic receptor. Methylmethacrylate monomer inhibits both intracellular calcium release and extracelluar calcium influx.

Keyword

methylmethacrylate monomer; rat; tracheal smooth muscle

MeSH Terms

Acetylcholine
Adenylyl Cyclases
Animals
Atropine
Baths
Calcium
Carbachol
Epithelium
Hip
Knee
Methylmethacrylate*
Muscle, Smooth
Muscle, Smooth, Vascular
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Orthopedic Procedures
Propranolol
Rats*
Receptors, Muscarinic
Relaxation
Tetraethylammonium
Trachea
Acetylcholine
Atropine
Calcium
Carbachol
Methylmethacrylate
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Propranolol
Receptors, Muscarinic
Tetraethylammonium