Korean J Fertil Steril.
1999 Aug;26(2):193-201.
Fertilization and in vitro Development of Bovine Oocytes Following Round Spermatid Injection
Abstract
- SUMMARY: In this study we determined fertilization processes and developmental ability of porcine oocytes following injection of round spermatid in the presence of artificial activation. Electrical stimulation at 3 h before spermatid injection significantly increased the incidence of normal fertilization as compared to those following injection without stimulation or with stimulation immediately after injection. The incidences of two pronuclear formation and apposition were not different in oocytes between following intracytoplasmic spermatid and spermatic nucleus injection. Indirect immunocytochemistry and laser scanning confocal microscopy study revealed that microtubules were organized from the oocyte cortex following round spermatid injection, and this seemed to move both male and female pronuclel into the oocyte center. Paternal mitochondria which are introduced with spermatid have been observed up to 4-cell. Our study indicated that either round spermatid or it's nucleus can be used to produce viable bovine embryos by injection into unfertilized oocytes.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Pregnancy and Delivery by Round Spermatid injection into Oocytes in Human
- Comparison of embryonic competence and clinical outcomes between early and late cumulus cell removal for in vitro fertilization
- In vitro fertilization and development of partial zona dissection and zona drilled mouse oocytes
- Microtubule and Chromatic Organization in Bovine Oocytes following Intracytoplasmic Injection of Spermatozoon, Sperm Head and Tail
- Clinical Usefulness of Intracytoplasmic Sperm Injection of 1-day-old Unfertilized Oocyte during IVF-ET
