Korean J Clin Pharm.  2016 Mar;26(1):70-76. 10.0000/kjcp.2016.26.1.70.

A Study on the Statistical Method for the Target Component of a Glucose-lowering Functional Material in Extracts of Evening Primerose Seeds

Affiliations
  • 1Department of Food and Drug, Chosun University Graduate School, Gwangju 61452, Republic of Korea. wlee@chosun.ac.kr
  • 2Graduate School of Clinical Pharmacy, Sookmyung Women's University, Seoul 04310, Republic of Korea.
  • 3College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea.

Abstract

BACKGROUND
The use of the extracts from evening primrose seeds as a health functional food has been gradually increased. Therefore, the monitoring and screening process has been considerably required for its quality control.
OBJECTIVE
This study aimed to estimate the measurement uncertainty associated with determination of penta-o-galloyl β-D-glucose (PGG) in extracts from evening primrose seeds by high-performance liquid chromatography.
METHODS
The sources of measurement uncertainty was expressed in accordance with mathematical/statistical theories of GUM & EURACHEM Guide. The expanded uncertainty was calculated by using the relative standard uncertainty between analytical result and sources of uncertainty in measurement (sample weight, final volume, extraction volume, standard solution, matrix and instrument etc).
RESULTS
In the results of 95% confidence interval, the uncertainty in measurement was 10,253.34 ± 1,844.50 µg/kg (k = 2.0).
CONCLUSION
In this study, it showed that the value of uncertainty in measurement for determination of PGG in extracts from evening primrose seeds by HPLC has about 18.0% influence on PGG contents of the analytical results. The results would be very useful for the monitoring and screening of evening primrose seeds marketed in Korea for its quality control as dietary supplement.

Keyword

Evening primrose seeds; quality control; penta-o-galloyl β-D-glucose; uncertainty measurement

MeSH Terms

Chromatography, High Pressure Liquid
Chromatography, Liquid
Dietary Supplements
Functional Food
Gingiva
Korea
Mass Screening
Oenothera biennis
Prostaglandins G
Quality Control
Uncertainty
Prostaglandins G
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