Korean Circ J.  2008 Aug;38(8):405-410. 10.4070/kcj.2008.38.8.405.

Polymorphism of the Angiotensin II Type 1 Receptor A1166C in Korean Hypertensive Adolescents

• Lee JA
• Sohn JA
• Hong YM

• KMID: 2225760
• DOI: http://doi.org/10.4070/kcj.2008.38.8.405

Abstract

BACKGROUND AND OBJECTIVES: The renin-angiotensin system (RAS) is a major regulator of blood pressure. The angiotensin II receptor type 1 (AGTR 1) A1166C has been extensively studied in searching for their involvement in the development of hypertension. The aim of this study was to determine the association of the AGTR 1 A1166C marker with essential hypertension in Korean adolescents.
SUBJECTS AND METHODS
Forty hypertensive adolescents were included in this study. The obesity index (OI) and body mass index (BMI) of the subjects were calculated. Blood pressure was measured at the resting state by oscillometric methods. The serum aldosterone, renin, insulin, angiotensin converting enzyme (ACE), homocysteine, vitamin B12 and folate levels were measured. The carotid intima-media thickness (IMT) and diameter and the brachial-ankle pulse wave velocity (baPWV) were evaluated by ultrasound. Polymerase chain reaction (PCR) was conducted to amplify the DNA of each of the study subjects to analyze the polymorphism of AGTR 1 A1166C.
RESULTS
The genotypic frequency of AA was 87.5%, that for adenylate cyclase (AC) was 12.5% and no CC type was detected. The serum homocysteine level was higher in the subjects with the AC genotype than that in the subjects with the AA genotype (11.9+/-2.9 umol/L vs 17.1+/-4.2 umol/L, respectively). The carotid IMT of the subjects with the AA genotype was greater than that of the subjects with the AC genotype (5.0+/-0.1 mm vs 8.0+/-0.2 mm, respectively).
CONCLUSION
In conclusion, the A1166C mutation group had a significantly greater carotid IMT and higher homocysteine levels than the group with the normal genotype of AGTR 1. The AC genotype of A1166C may be useful to predict the presence of early coronary artery disease in hypertensive adolescents. More investigation is necessary to clarify the relation between the A1166C gene and its involvement with coronary artery disease in hypertensive Korean adolescents.

Keyword

Angiotensins; Hypertension; Genes; Adolescent

MeSH Terms

Adenylyl Cyclases
Adolescent
Aldosterone
Angiotensin II
Angiotensins
Blood Pressure
Body Mass Index
Carotid Intima-Media Thickness
Coronary Artery Disease
DNA
Folic Acid
Genotype
Homocysteine
Humans
Hypertension
Insulin
Obesity
Peptidyl-Dipeptidase A
Polymerase Chain Reaction
Pulse Wave Analysis
Receptor, Angiotensin, Type 1
Receptors, Angiotensin
Renin
Renin-Angiotensin System
Vitamin B 12
Aldosterone
Angiotensin II
Angiotensins
DNA
Folic Acid
Homocysteine
Insulin
Peptidyl-Dipeptidase A
Receptor, Angiotensin, Type 1
Receptors, Angiotensin
Renin
Vitamin B 12

Figure

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  Fig. 1 Angiotensin II type 1 receptor A1166C gene polymorphism. The allele C produced two bands at 427 and 110 bp, while the A allele remained undigested. Lane M: DNA size, Lane 1: A/A, Lane 2: A/A, Lane 3: A/C, A: 527 bp, C: 417 bp+110 bp.

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