Korean J Clin Pathol.  1997 Apr;17(2):287-295.

Detection of Human Papillomavirus in Uterine Cervical Cancer Tissues by Polymerase Chain Reaction, In Situ Hybridization and Polymerase Chain Reaction In Situ Techniques

• Kim SC
• Suh SP
• Kee SJ
• Shin MG
• Byun DE
• Shin JH
• Song JW
• Park CS
• Choi HS
• Ryang DW

Abstract

BACKGROUND: Human papillomavirus (HPV) is a small double-stranded DNA virus. Of HPV, type 16 and 18 are associated with high risk in the development of cervical cancer. In order to evaluate HPV infections, several HPV typing and detection methods have been developed. The aim of this study was to compare the detection rates of HPV 16 and 18 by polymerase chain reaction (PCR), in situ hybridization(ISH), and PCR in situ in uterine cervical cancers.
METHODS
PCR, ISH and PCR in situ were performed for the detection of HPV DNA in fifty-one formalin fixed, paraffin embedded blocks of uterine cervical cancer tissues. Twenty uterine cervical specimens from patients with uterine myomas were used as controls.
RESULTS
The detection rates of HPV 16 and HPV 18 in cervical cancers were 56.9% (29/51) and 45.1% (23/51) by PCR, 9.8% (5/51) and 5.9% (3/51) by ISH, 17.6% (9/51) and 11.8% (6/51) by PCR in situ, respectively. In control group, the detection rate of HPV 16 and 18 by PCR were 10% (2/20) and 5% (1/20), but HPV was not detected by both ISH and PCR in situ.
CONCLUSION
PCR was the most sensitive method for the detection of HPV. However, PCR in situ was more informative fort the specific detection and cell localization of HPV DNA.

MeSH Terms

DNA
Formaldehyde
Human papillomavirus 16
Human papillomavirus 18
Humans*
In Situ Hybridization*
Leiomyoma
Paraffin
Polymerase Chain Reaction*
Uterine Cervical Neoplasms*
DNA
Formaldehyde
Paraffin