Korean J Clin Microbiol.
2005 Apr;8(1):17-25.
Prevalence of Ambler Class A Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea
- Affiliations
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- 1Department of Pediatrics, Kosin University College of Medicine, Busan, Korea.
- 2Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea. kscpjsh@ns.kosinmed.or.kr
- 3Department of Laboratory Medicine, Keonyang University College of Medicine, Daejeon, Korea.
- 4Department of Laboratory Medicine, Ajou University College of Medicine, Suwon, Korea.
- 5Department of Laboratory Medicine, Hanyang University College of Medicine, Guri, Korea.
- 6Department of Laboratory Medicine, Sooncheonhyang University College of Medicine, Gumi, Korea.
- 7Department of Laboratory Medicine, Pochon Cha University College of Medicine, Sungnam, Korea.
- 8Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
- 9Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
- 10Department of Laboratory Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.
- 11Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
- 12Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
- 13Division of Food Microbiology, Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea.
Abstract
- BACKGROUND
The aim of this study is to determine the nationwide prevalence of Ambler class A extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and to characterize genotypes of ESBLs.
METHODS
During the period of February through July, 2003, E.coli and K.pneumoniae isolates were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were tested by disk diffusion method, and ESBL-production was determined by the double-disk synergy test. MICs of beta-lactam antibiotics were tested by agar dilution method. Searches for bla TEM, bla SHV, bla CTX-M, bla PER-1, bla VEB, bla IBC, bla GES and bla TLA genes were performed by PCR amplification, and the genotypes of ESBLs were determined by direct nucleotide sequence analysis of amplified products.
RESULTS
Resistance rates of E.coli (n=246) and K.pneumoniae (n=239) isolates to ceftazidime were 8.5% and 20.1%, respectively. Most prevalent Ambler class A ESBL genotypes in E.coli isolates were bla CTX-M-15 (n=4) and bla CTX-M-3 (n=3), and each of bla CTX-M-14, bla SHV-12, and bla TEM-52 gene was also found in one isolate. Most prevalent ESBL genotypes in K.pneumoniae were bla SHV-12 (n=30) and bla CTX-M-3 (n=13), and bla CTX-M-14 (n=5). bla SHV-2a (n=3), bla SHV-5 (n=2), bla TEM-52 (n=1), bla GES-3 (n=2) genes were also found.
CONCLUSION
CTX-M-type ESBL-producing E.coli and K.pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
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