Korean J Anat.  2000 Oct;33(5):529-541.

Immunohistochemical Reaction of Calcitonin-gene-related Peptide and Type II Collagen and Morphological Changes of Cartilage Implants and Cultured Chondrocytes

Affiliations
  • 1Department of Anatomy, College of Medicine, Inje University, Korea.
  • 2Department of Anatomy, College of Medicine, Kosin University, Korea.

Abstract

The purpose of this study was to investigate the immunohistochemical reaction of calcitonin-gene-related peptide (CGRP) and type II collagen and also morphological changes of cartilage implants and cultured chondrocytes isolated from the articular and costal cartilages. The chondrocytes were isolated from the head of the femur and the 11th costal cartilage of the 6 months old rabbits. De novo implants were prepared from the chondrocytes cultured on the perichondrium by culturing isolated articular chondrocytes. Cultured chondrocytes and implants were evaluated by immunohistochemical staining of CGRP and type II collagen and electron microscopy. Articular chondrocytes maintained the typical phenotype in the 1st and 2nd subcultures, but the costal chondrocytes were transformed into fibroblast-like cells. The articular chondrocytes cultured on the perichondrium were more flattened and formed the cartilage. Most chondrocytes were no loss of type II collagen immunostaining by culturing. Implants replaced by the cultured articular chondrocytes were generally increased CGRP and decreased type II collagen immunoreaction. Electron microscopically the cultured articular chondrocytes had a large euchromatic nucleus, a few granules, and abundant vesicles. During culture, the nucleus became atropy and the cytoplasm contained many large vacuoles. The chondrocytes cultured on the perichondrium showed a lot of segmented rough endoplasmic reticulum and fine short microvilli. During culture, articular chondrocytes maintained typical phenotype and type II collagen reaction. The cultured articular chondrocytes had some organelles and euchromatic nucleus with prominent nucleolus. The chondrocytes cultured on the perichondrium showed active secretion of the matrix with small vesicles and well developed endoplasmic reticulum. The implanted articular chondrocytes showed the decrease of their organelles after secretion of the marix and became increased CGRP and decreased type II collagen immunoreaction.

Keyword

Implanted articular chondrocytes; CGRP; Type II collagen

MeSH Terms

Cartilage*
Chondrocytes*
Collagen Type II*
Cytoplasm
Endoplasmic Reticulum
Endoplasmic Reticulum, Rough
Femur
Head
Humans
Infant
Microscopy, Electron
Microvilli
Organelles
Phenotype
Rabbits
Vacuoles
Collagen Type II
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