J Korean Soc Vasc Surg.  2005 Nov;21(2):113-117.

Angiogenesis Induced by the Implantation of Autogenous Whole Bone Marrow Stem Cells in an Ischemic Animal Model

• Kim DI
• Im RJ
• Lim JE
• Kim JS
• Jeon HJ
• Jang IS
• Kim BS
• Cho SW

Abstract

PURPOSE
Bone marrow contains many kinds of primitive cells and endothelial progenitor cells that secrete several growth factors. We hypothesized that angiogenesis could be induced by autogenous whole bone marrow stem cell implantation in an animal ischemic limb. METHOD: A chronic ischemic hind limb model was created by encircling the femoral artery with an ameroid constrictor (2 mm inner diameter) in a dog model. About 20 ml of autogenous whole bone marrow stem cells were aspirated from the femur and then injected into ischemic limb muscles. Contralateral limbs injected with 20 ml of normal saline as controls. To assess angiogenic effects, an angiogram and a histologic evaluation were performed at 8 weeks after bone marrow stem cell implantation. RESULT: Imaging analysis of angiograms showed that newly developed capillaries were significantly more plentiful in treated limbs. Mean capillary density in the treated limb group was significantly greater than that in the control group (151+/-11.7 vs 81.5+/-7.2 cap/mm2, respectively, P<0.05), and the proportion of larger diameter (Fig. 6) newly developed capillaries was significantly higher in treated limbs than in control limbs.
CONCLUSION
These findings indicated that autogenous whole bone marrow stem cell implantation increases the efficiency of angiogenesis.

Keyword

Angiogenesis; Stem cell; Bone marrow

MeSH Terms

Animals*
Bone Marrow*
Capillaries
Dogs
Extremities
Femoral Artery
Femur
Intercellular Signaling Peptides and Proteins
Models, Animal*
Muscles
Stem Cells*
Intercellular Signaling Peptides and Proteins