J Neurogastroenterol Motil.
2016 Apr;22(2):310-320. 10.5056/jnm15082.
Changes in Enteric Neurons of Small Intestine in a Rat Model of Irritable Bowel Syndrome with Diarrhea
- Affiliations
-
- 1Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. fangxiucai2@aliyun.com
- 2Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China (Current address).
- 3Department of Physiology and Cell Biology, The Ohio State University, Wexner Medical Center, Columbus, USA.
- KMID: 2203585
- DOI: http://doi.org/10.5056/jnm15082
Abstract
- BACKGROUND/AIMS
Physical and/or emotional stresses are important factors in the exacerbation of symptoms in irritable bowel syndrome (IBS). Several lines of evidence support that a major impact of stress on the gastrointestinal tract occurs via the enteric nervous system. We aimed to evaluate histological changes in the submucosal plexus (SMP) and myenteric plexus (MP) of the distal ileum in concert with the intestinal motor function in a rat model of IBS with diarrhea.
METHODS
The rat model was induced by heterotypic chronic and acute stress (CAS). The intestinal transit was measured by administering powdered carbon by gastric gavage. Double immunohistochemical fluorescence staining with whole-mount preparations of SMP and MP of enteric nervous system was used to assess changes in expression of choline acetyltransferase, vasoactive intestinal peptide, or nitric oxide synthase in relation to the pan neuronal marker, anti-Hu.
RESULTS
The intestinal transit ratio increased significantly from control values of 50.8% to 60.6% in the CAS group. The numbers of enteric ganglia and neurons in the SMP were increased in the CAS group. The proportions of choline acetyltransferase- and vasoactive intestinal peptide-immunoreactive neurons in the SMP were increased (82.1 ± 4.3% vs. 76.0 ± 5.0%, P = 0.021; 40.5 ± 5.9% vs 28.9 ± 3.7%, P = 0.001), while nitric oxide synthase-immunoreactive neurons in the MP were decreased compared with controls (23.3 ± 4.5% vs 32.4 ± 4.5%, P = 0.002).
CONCLUSIONS
These morphological changes in enteric neurons to CAS might contribute to the dysfunction in motility and secretion in IBS with diarrhea.
MeSH Terms
-
Animals
Carbon
Choline
Choline O-Acetyltransferase
Diarrhea*
Enteric Nervous System
Fluorescence
Ganglia
Gastrointestinal Motility
Gastrointestinal Tract
Ileum
Intestine, Small*
Irritable Bowel Syndrome*
Models, Animal*
Myenteric Plexus
Neurons*
Nitric Oxide
Nitric Oxide Synthase
Rats*
Stress, Psychological
Submucous Plexus
Vasoactive Intestinal Peptide
Carbon
Choline
Choline O-Acetyltransferase
Nitric Oxide
Nitric Oxide Synthase
Vasoactive Intestinal Peptide
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