J Korean Soc Transplant.  2001 Dec;15(2):130-133.

The Effect of Lipo-PGE1 in Canine Partial Liver Allograft Model

• Chung KY
• Kim YS
• Chae YS
• Lee WJ
• Kim MS
• Choi KJ

Abstract

PURPOSE: Hepatoprotective effect of prostaglandin E1 (PGE1) has been verified in numerous animal experiments but not so apparent in clinical trials. Although the reason for this discrepancy in clinical results is still unknown, one possible explanation is the instability of PGE1. In this study, the hepatoprotective effect of lipo-PGE1, which is known to be stable during pulmonary circulation and have more targeting effect, was investigated in canine partial liver allotansplantation. In order to reckon in the possible injury during harvest of partial liver, lipo-PGE1 was infused from the start of living graft harvest procedure.
METHODS
Mongrel dogs weighing about 25 kg were divided into control (n=6) and lipo-PGE1 (n=6) group. Partial liver allotransplantation was performed. In lipo-PGE1 group, lipo-PGE1 was slowly infused through splenic venous cannulation during the donor liver harvesting procedure (50 mg) and continuously infused (60 mg/day) for 48 hrs after reperfusion. The aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were monitored.
RESULTS
The AST and ALP levels of the lipo-PGE1 group were significantly lower than that of the control group at both 1 hour and 48 hours after reperfusion. The LDH level in lipo-PGE1 group was lower at 1 hour and 48 hours after reperfusion, but no significant differences were shown between two groups.
CONCLUSION
This study demonstrated the hepatoprotective effect of the lipo-PGE1 against ischemia-reperfusion injury in canine partial liver allotransplantation.

Keyword

Ischemic-reperfusion injury; Liver transplantation; Lipo-PGE1

MeSH Terms

Alkaline Phosphatase
Allografts*
Alprostadil*
Animal Experimentation
Animals
Aspartate Aminotransferases
Catheterization
Dogs
Humans
L-Lactate Dehydrogenase
Liver Transplantation
Liver*
Pulmonary Circulation
Reperfusion
Reperfusion Injury
Tissue Donors
Transplants
Alkaline Phosphatase
Alprostadil
Aspartate Aminotransferases
L-Lactate Dehydrogenase