J Korean Acad Prosthodont.  2009 Apr;47(2):232-239. 10.4047/jkap.2009.47.2.232.

Evaluation of changes in adhesive strength and cytotoxicity of a denture adhesive according to time

• Jung HY
• Kim JH
• Lee KW
• Shim JS
• Moon HS

• KMID: 2195621
• DOI: http://doi.org/10.4047/jkap.2009.47.2.232

Abstract

STATEMENTS OF THE PROBLEM: Many denture wearers occasionally use denture adhesives to improve denture retention, stability and chewing efficiency. An ideal denture adhesive is nontoxic, non-irritating, and provides comfort to the oral mucosa. PURPOSE: The purpose of this study was to evaluate the cytotoxicity and adhesive properties of a selected denture adhesive. MATERIAL AND METHODS: To test cytotoxicity of the selected denture adhesive, mouse fibroblast cells were used in MTT testing. Cytotoxicity was examined according to the concentration of the denture adhesive and incubated for 1 to 4 days. To examine adhesive property, a denture base was fabricated on an edentulous dentiform. The adhesive was applied to the denture base, then tensile bond strength was measured, to evaluate the change in retention during 3 days. RESULTS AND CONCLUSION: 1. 1% and 2% concentration denture adhesive cream had no cytotoxicity. 2. The tensile bond strength of the group with both denture adhesive and artificial saliva was significantly higher than that of the group with only denture adhesive (P < .05). The tensile bond strength of the group with denture adhesive was significantly higher than that of with only artificial saliva (P < .05). 3. The tensile bond strength had no significant change during 1 hour, and then gradually decreased. After 1 day, it decrease to half. Within the limitation of this study, the tested denture adhesive had no cytotoxicilty and was effective in improving denture retention. The adhesive strength began to continuously decrease after 1 hour and it decreased to half at 1 day after application.

Keyword

denture adhesive; tensile bond strength; cytotoxicity; MTT test

MeSH Terms

Adhesives
Animals
Dental Restoration Wear
Denture Bases
Denture Retention
Dentures
Fibroblasts
Mastication
Mice
Mouth Mucosa
Retention (Psychology)
Saliva, Artificial
Adhesives
Saliva, Artificial

Figure

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  Fig. 1. Dentiform and denture base used to test tensile bond strength of denture adhesive.

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  Fig. 2. Position of the test specimen for measurement of tensile bond strength.

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  Fig. 3. Change of optical density at time and concentration.

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  Fig. 4. Tensile bond strength of saliva (S), adhesive (A), adhesive and saliva (A + S).

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  Fig. 5. Tensile bond strength (N) change according to time.

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