J Korean Neurosurg Soc.  2012 Jun;51(6):328-333. 10.3340/jkns.2012.51.6.328.

Increased Vascular Endothelial Growth Factor in the Ventricular Cerebrospinal Fluid as a Predictive Marker for Subsequent Ventriculoperitoneal Shunt Infection : A Comparison Study among Hydrocephalic Patients

• Lee JH
• Back DB
• Park DH
• Cha YH
• Kang SH
• Suh JK

• KMID: 2190485
• DOI: http://doi.org/10.3340/jkns.2012.51.6.328

Abstract

OBJECTIVE
The aim of this study is to determine the association between the cerebrospinal fluid (CSF) biomarkers and inflammation, and the predictive value of these CSF biomarkers for subsequent shunt associated infection.
METHODS
We obtained CSF samples from the patients with hydrocephalus during ventriculoperitoneal (VP) shunt operations. Twenty-two patients were enrolled for this study and divided into 3 groups: subarachnoid hemorrhage (SAH)-induced hydrocephalus, idiopathic normal pressure hydrocephalus (INPH) and hydrocephalus with a subsequent shunt infection. We analyzed the transforming growth factor-beta1, tumor necrosis factor-alpha, vascular endothelial growth factor (VEGF) and total tau in the CSF by performing enzyme-linked immunosorbent assay. The subsequent development of shunt infection was confirmed by the clinical presentations, the CSF parameters and CSF culture from the shunt devices.
RESULTS
The mean VEGF concentration (+/-standard deviation) in the CSF of the SAH-induced hydrocephalus, INPH and shunt infection groups was 236+/-138, 237+/-80 and 627+/-391 pg/mL, respectively. There was a significant difference among the three groups (p=0.01). Between the SAH-induced hydrocephalus and infection groups and between the INPH and infection groups, there was a significant difference of the VEGF levels (p<0.01). However, the other marker levels did not differ among them.
CONCLUSION
The present study showed that only the CSF VEGF levels are associated with the subsequent development of shunt infection. Our results suggest that increased CSF VEGF could provide a good condition for bacteria that are introduced at the time of surgery to grow in the brain, rather than reflecting a sequel of bacterial infection before VP shunt.

Keyword

Cerebrospinal fluid; Shunt infection; Biomarkers; Vascular endothelial growth factor; Hydrocephalus

MeSH Terms

Bacteria
Bacterial Infections
Biomarkers
Brain
Enzyme-Linked Immunosorbent Assay
Humans
Hydrocephalus
Hydrocephalus, Normal Pressure
Inflammation
Subarachnoid Hemorrhage
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A
Ventriculoperitoneal Shunt
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A

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