J Korean Soc Neonatol.  1999 May;6(1):1-7.

Purification and Analysis of Amino Acid Sequences of Pulmonary Surfactant Proteins

• Bae CW
• Choi YM

Abstract

PURPOSE: For the synthesis of surfactant protein(SP) peptides and production of next generation artificial pulmonary surfactant(PS), we have isolated SP-B, C from bovine PS, and studied the biochemical properties and amino acid sequences of these protein-peptides.
METHODS
Crude surfactant and purified surfactant were isolated from materials extracted from the bovine lung lavage. The hydrophobic SP-B, C were purified by Sephadex LH 60 column chromatography from PS. The purities of SP-B, C were assessed by tricine buffer SDS-polyacrylamide gel electrophoresis and the amino acid sequences of these proteins were determined using Beckman PI-2090.
RESULTS
The molecular weights of SP-B, C shown in SDS-polyacrylamide gel electrophoresis were as follows; 15,000-18,000(oligomer) Da for SP-B, 3,500-5,000 Da for SP-C. The amino acid sequences were; FPIPLPYCWL LRTLIKKIQA VIPKGVLAMT VAQCHVVPL LVGGIQQLV IEYSVILLTD TLLGRLPNLV CGLRLRCSG in SP-B, LIPCCPVNIK RLLIVVVVVV LLVVVIVGAL LMGL in SP-C, respectively. These results indicated that the amino acid sequences of bovine SPs were different.
CONCLUSION
The SP-B, C were purified from bovine PS, and amino acid sequences of SP-B, C were determined. Further studies are needed for the development and use of next generations of exogenous PS preparation based on synthetic SP-peptides for the treatment of neonatal RDS in the future.

Keyword

Pulmonary surfactant; Surfactant protein(SP); SP-B, C, Peptides; Respiratory distress syndrome(RDS); Newborn infants

MeSH Terms

Amino Acid Sequence*
Bronchoalveolar Lavage
Chromatography
Electrophoresis
Family Characteristics
Humans
Infant, Newborn
Molecular Weight
Peptides
Pulmonary Surfactants*
Peptides
Pulmonary Surfactants