J Adv Prosthodont.  2014 Feb;6(1):14-21. 10.4047/jap.2014.6.1.14.

Investigation of anodized titanium implants coated with triterpenoids extracted from black cohosh: an animal study

• Park IP
• Kang TJ
• Heo SJ
• Koak JY
• Kim JH
• Lee JH
• Lee SJ
• Kim SK

• KMID: 2176565
• DOI: http://doi.org/10.4047/jap.2014.6.1.14

Abstract

PURPOSE
The purpose of this study was to evaluate bone response to anodized titanium implants coated with the extract of black cohosh, Asarum Sieboldii, and pharbitis semen.
MATERIALS AND METHODS
Forty anodized titanium implants were prepared as follows: group 1 was for control; group 2 were implants soaked in a solution containing triterpenoids extracted from black cohosh for 24 hours; group 3 were implants soaked in a solution containing extracts of black cohosh and Asarum Sieboldii for 24 hours; group 4 were implants soaked in a solution containing extracts of pharbitis semen for 24 hours. The implants from these groups were randomly and surgically implanted into the tibiae of ten rabbits. After 1, 2, and 4 weeks of healing, the nondecalcified ground sections were subjected to histological observation, and the percentage of bone-to-implant contact (BIC%) was calculated.
RESULTS
All groups exhibited good bone healing with the bone tissue in direct contact with the surface of the implant. Group 2 (52.44 +/- 10.98, 25.54 +/- 5.56) showed a significantly greater BIC% compared to that of group 3 (45.34 +/- 5.00, 22.24 +/- 2.20) with respect to the four consecutive threads and total length, respectively. The BIC% of group 1 (25.22 +/- 6.00) was significantly greater than that of group 3 (22.24 +/- 2.20) only for total length.
CONCLUSION
This study did not show any remarkable effects of the extract of black coshosh and the other natural products on osseointegration of anodized titanium implants as coating agents. Further studies about the application method of the natural products on to the surface of implants are required.

Keyword

Titanium implant; Triterpenoid; Black cohosh; Pharbitis semen; Asarum Sieboldii; Bone-implant contact

MeSH Terms

Animals*
Asarum
Biological Products
Bone and Bones
Cimicifuga*
Methods
Osseointegration
Rabbits
Semen
Tibia
Titanium*
Biological Products
Titanium

Figure

• 

  Fig. 1 The implants were installed in the proximal aspect of each tibia.

• 

  Fig. 2 Model of the histomorphometric analysis.

• 

  Fig. 3 Light microscope view (toluidine blue; original magnification ×100) at 1 week: (A) control group; (B) group 2; (C) group 3; (D) group 4. Endosteal bone surfaces showed signs of bone formation (arrow) by appositional growth which sometimes reached the implant surface in all groups.

• 

  Fig. 4 Light microscope views (toluidine blue; original magnification ×100) at two weeks: (A) control group; (B) group 2; (C) group 3; (D) group 4. And at four weeks: (E) control group; (F) group 2; (G) group 3; (H) group 4.

• 

  Fig. 5 (A) The percentages of bone-to-implant contact in the four consecutive threads. Group 2 showed a significantly greater BIC% than did group 3 (P<.05). (B) Groups 1 and 2 showed significantly greater BIC%s than did group 3 with respect to total length (P<.05).

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