J Korean Acad Conserv Dent.  2008 Jul;33(4):369-376. 10.5395/JKACD.2008.33.4.369.

Ingredients and cytotoxicity of MTA and 3 kinds of Portland cements

• Chang SW
• Yoo HM
• Park DS
• Oh TS
• Bae KS

• KMID: 2176004
• DOI: http://doi.org/10.5395/JKACD.2008.33.4.369

Abstract

The aim of this study was to compare the compositions and cytotoxicity of white ProRoot MTA (white mineral trioxide aggregate) and 3 kinds of Portland cements. The elements, simple oxides and phase compositions of white MTA (WMTA), gray Portland cement (GPC), white Portland cement (WPC) and fast setting cement (FSC) were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES), X-ray fluorescence spectrometry (XRF) and X-ray diffractometry (XRD). Agar diffusion test was carried out to evaluate the cytotoxicity of WMTA and 3 kinds of Portland cements. The results showed that WMTA and WPC contained far less magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) than GPC and FSC. FSC contained far more aluminum oxide (Al2O3) than WMTA, GPC, and WPC. WMTA, GPC, WPC and FSC were composed of main phases, such as tricalcicium silicate (3CaO.SiO2), dicalcium silicate (2CaO.SiO2), tricalcium aluminate (3CaO.Al2O3), and tetracalcium aluminoferrite (4CaO.Al2O3.Fe2O3). The significance of the differences in cellular response between WMTA, GPC, WPC and FSC was statistically analyzed by Kruskal-Wallis Exact test with Bonferroni's correction. The result showed no statistically significant difference (p > 0.05). WMTA, GPC, WPC and FSC showed similar compositions. However there were notable differences in the content of minor elements, such as aluminum (Al), magnesium, iron, manganese, and zinc. These differences might influence the physical properties of cements.

Keyword

White MTA (white mineral trioxide aggregate); Portland cement; ICP-AES (Inductively coupled plasma atomic emission spectrometry); XRF (X-ray fluorescence spectrometry); XRD (X-ray diffractometry); Agar diffusion test

MeSH Terms

Agar
Aluminum
Aluminum Oxide
Calcium Compounds
Diffusion
Glutamates
Guanine
Humans
Iron
Magnesium
Manganese
Oxides
Plasma
Silicates
Spectrometry, X-Ray Emission
Spectrum Analysis
Zinc
Pemetrexed
Agar
Aluminum
Aluminum Oxide
Calcium Compounds
Glutamates
Guanine
Iron
Magnesium
Manganese
Oxides
Silicates
Zinc

Figure

• 

  Figure 1 X-ray diffraction (XRD) analysis of white MTA (a) and Portland cement (b) showing the main phase (2CaO·SiO2) present in the cement. (MTA: mineral trioxide aggregate) (Black : peaks made by specimen, Red : peaks of reference)

• 

  Figure 2 Cellular response to WMTA, GPC, WPC, and FSC, 6 hrs after placement of specimens. (WMTA : white MTA, GPC : Gray Portland cement, WPC : white Portland cement, and FSC : Fast setting cement, ZOE : Zinc Oxide and Euginol)

• 

  Figure 3 Schematic diagram showing the compositions of WMTA and Portland cements. (WMTA : white mineral trioxide aggregate)

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