J Korean Acad Conserv Dent.  2009 May;34(3):192-198. 10.5395/JKACD.2009.34.3.192.

Comparison of biocompatibility of four root perforation repair materials

• Kang MK
• Bae IH
• Koh JT
• Hwang YC
• Hwang IN
• Oh WM

• KMID: 2176144
• DOI: http://doi.org/10.5395/JKACD.2009.34.3.192

Abstract

This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay. The results of SEM revealed the cells in contact with GIC, MTA, and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA.

Keyword

Dycal; Glass-ionomer cement; MTA; Portland cement; Biocompatibility; Cytotoxicity

MeSH Terms

Acrylic Resins
Aluminum Compounds
Calcium Compounds
Calcium Hydroxide
Drug Combinations
Glass Ionomer Cements
Glutamates
Guanine
Humans
Hydroxides
Microscopy, Electron, Scanning
Minerals
Oxides
Silicates
Silicon Dioxide
Pemetrexed
Acrylic Resins
Aluminum Compounds
Calcium Compounds
Calcium Hydroxide
Drug Combinations
Glass Ionomer Cements
Glutamates
Guanine
Hydroxides
Minerals
Oxides
Silicates
Silicon Dioxide

Figure

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  Figure 1 Cells after the incubation with test material for 1 day (×250, a: Dycal, b: GIC, c: MTA, d: Portland cement)

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  Figure 2 Cell viability after 1 day, 3 day, 8 day incubation with the extract from Dycal, GIC, MTA, and Portland cement as measured by XTT assay (a: 1 day extract, b: 4 day extract, C: 7 day extract), (+)CTR : Positive control) *Significantly difference (p < 0.05) between the materials.

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