J Korean Cancer Assoc.  1998 Feb;30(1):12-19.

p53 Mutation of Head and Neck Squamous Cell Carcinoma Cell Lines

Affiliations
  • 1Department of Otorhinolaryngology, College of Medicine, Sung Kyun Kwan University, Samsung Medical Center.

Abstract

PURPOSE
Structural alterations of p53 and overexpression of the p53 protein are the most common genetic abnormalities in various kinds of human cancers. In this study, we examined the mutational status and the frequency of p53 mutations in head and neck squamous cell carcimona (HNSCC) cell lines.
MATERIALS AND METHODS
7 human head and neck squamous cell carcinoma cell lines were included in this analysis. Using polymerase chain reaction(PCR), single strand confonmation polymorphism(SSCP) and PCR-DNA sequencing analysis, we tested the mutational status of 7 cell lines. Exon 4~9 of the p53 gene was amplified for the direct DNA sequencing analysis.
RESULTS
Our results showed 100% nuclear p53 immunostaining and 3 electrophoretic abnomalities by PCR-SSCP in three cancer cell lines and mutations of the p53 gene including 2 base substitutions and 1 base deletion were detected in 3 cancer cell lines using PCR directed DNA sequencing analysis.
CONCLUSION
7 HNSCC cell lines examined in this study provide excellent systems for study of gene therapy using p53 gene.

Keyword

p53 mutations; HNSCC; PCR-SSCP; Sequencing

MeSH Terms

Carcinoma, Squamous Cell*
Cell Line*
Exons
Genes, p53
Genetic Therapy
Head*
Humans
Neck*
Polymerase Chain Reaction
Sequence Analysis, DNA
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