Intest Res.  2009 Jun;7(1):32-40.

Expression of Matrix Metallopreoteinases and Tissue Inhibitors of Metalloproteinases in Ulcerative Colitis

• Kim SE
• Jung SA
• Shim KN
• Jung HK
• Kim TH
• Yoo K

Abstract

BACKGROUND/AIMS
Ulcerative colitis (UC) is characterized by chronic non-specific inflammation in the mucosa and submucosa of the colon. Degradation of the extracellular matrix (ECM) is one of the major events during this process. Matrix metalloproteinases (MMPs) are important enzymes involved in the degradation of the ECM, and the activities of MMPs are controlled by its natural inhibitor, tissue inhibitor of metalloproteinases (TIMPs). This study was performed to determine the expression of MMPs and TIMPs in patients with UC.
METHODS
Twenty-nine patients with UC and 5 controls were included. Colonoscopic biopsies were obtained from the cecum, ascending colon, transverse colon, sigmoid colon, and rectum in each patient. The mRNA levels of expression of MMP-2 and -9, and TIMP-1 and -2 were measured separately using reverse transcription polymerase chain reactions in the mucosal specimens from each 5 segments of the colon.
RESULTS
The mRNA expression of MMP-2 and -9, and TIMP-1 in the inflamed tissues of patients with UC was significantly increased compared to non-inflamed tissues of patients with UC and controls (p<0.05). The mRNA expression of MMP-9 and TIMP-1 in non-inflamed tissues of patients with UC was significantly higher than that of controls (p<0.05). In inflamed tissues of UC, the mRNA expression of MMP-2 was significantly correlated with TIMP-2, and the mRNA expression of MMP-9 was significantly correlated with TIMP-1. The MMP-2/TIMP-2 ratio was increased in inflamed tissues compared to non-inflamed tissues of patients with UC and controls (p<0.05).
CONCLUSIONS
MMP-2 and-9, and TIMP-1 are likely to contribute to the inflammatory cascade in UC. MMP-2 and-9, and TIMP-1 might be important clues to solve the pathogenesis of UC.

Keyword

Matrix Metalloproteinases; Tissue Inhibitor of Metalloproteinases; Colitis, Ulcerative

MeSH Terms

Biopsy
Cecum
Colitis, Ulcerative
Colon
Colon, Ascending
Colon, Sigmoid
Colon, Transverse
Extracellular Matrix
Humans
Inflammation
Matrix Metalloproteinases
Metalloproteases
Mucous Membrane
Polymerase Chain Reaction
Rectum
Reverse Transcription
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinases
Ulcer
Matrix Metalloproteinases
Metalloproteases
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinases