Intest Res.  2008 Dec;6(2):128-134.

Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease:Comparing of Immunohistochemical Stain to Real-time PCR

• Park YS
• Kim NI
• Lee JS
• Choi JH
• Im CY
• Jun DW
• Jo YJ
• Kim SH
• Son BK
• Lee WM
• Kim EK

Abstract

BACKGROUND/AIMS: This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR). METHODS: Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR. RESULTS: Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa. CONCLUSIONS: Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining.

Keyword

Cyclooxygenase 2; Inflammatory Bowel Diseases; Immunohistochemistry; Polymerase Chain Reaction

MeSH Terms

Colitis
Colitis, Ulcerative
Colon
Crohn Disease
Cyclooxygenase 2
Humans
Immunohistochemistry
Inflammation
Inflammatory Bowel Diseases
Mucous Membrane
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Cyclooxygenase 2