J Korean Breast Cancer Soc.  2002 Jun;5(2):113-117. 10.4048/jkbcs.2002.5.2.113.

Cytologic Evaluation of p53, Cyclin D1, and Cathepsin D and Their Correlation with Histologic Sections in Primary Breast Carcinoma Original Paper

• Park K
• Han S

• KMID: 2173558
• DOI: http://doi.org/10.4048/jkbcs.2002.5.2.113

Abstract

PURPOSE: Biologic characteristics of the tumors could be altered after chemotherapy. Accurate assessment of tumor biology before treatment is important for selecting treatment modalities. Current study was designed to investigate whether multiple molecular markers could be accurately assayed on the fine needle aspirates from the breast carcinoma.
METHODS
Immunocytochemical assays (ICA) for p53, cyclin D1, and cathepsin D were performed on cytologic samples from 76 primary breast carcinomas, 37 ductal carcinoma in situ (DCIS), and 36 benign ductal hyperplasia. ICA for 3 molecules was also performed on the histologic sections from the matching tumor blocks, and the results were compared.
RESULTS
Three molecular markers were successfully detected in cytologic samples from the breast carcinomas; p53 in 71.1% (54/76), cyclin D1 in 73.7% (56/76), and cathepsin D in 44.7% (34/76). Their expression was rarely observed in benign hyperplasia; p53 in 0/36, cyclin D1 in 7/36, and cathepsin D in 4/36. Increase of expression frequency of 3 molecules was apparent through the progress of the disease. Results of ICA for each molecules were well correlated between cytologic and histologic samples; concordance was 93.4% (71/76) for p53, 81.6% (62/76) for cyclin D1, and 73.7% (56/76) for cathepsin D. When 3 molecular markers were integrated to the preoperative diagnosis, positive predictive value was 90.6% for malignant breast disease.
CONCLUSION
Three molecular markers were successfully assayed on cytologic samples and well correlated with the results from the histologic sections. The results indicate that the biologic information from the fine needle aspirates can be reliably integrated to patients treatment.

Keyword

Cathepsin D ; Cyclin D1 ; Cytology ; Diagnosis ; p53 ; Treatment

MeSH Terms

Biology
Breast Diseases
Breast Neoplasms*
Breast*
Carcinoma, Intraductal, Noninfiltrating
Cathepsin D*
Cathepsins*
Cyclin D1*
Cyclins*
Diagnosis
Drug Therapy
Humans
Hyperplasia
Needles
Population Characteristics
Cathepsin D
Cathepsins
Cyclin D1
Cyclins