Ewha Med J.  2004 Mar;27(1):33-39. 10.12771/emj.2004.27.1.33.

Marker-Genes for Dioxin-Induced Hematopoietic Stem Cell Dysfuction Using the Oligonucleotide-Microarray in Mice

Affiliations
  • 1Department of Biochelmistry, College of Medicine, Ewha Womans University, Korea.

Abstract

To develop the biomarkers for the immune dysfunction induced by dioxin, 2, 3, 7, 8-TCDD was administered C57BL/6N mice 0.1microg TCDD/??body weight. Colony forming assay showed that the proliferation potential of hemotopoieti progenitor cells in bone marrow was reduced 35-55% more by earlier exposure. The microarray experiments were duplicated, and the candidates were restricted for the genes expressed greater than 121-fold. Resulting candidates were 55 genes. The expression patterns of the whole genes were analyzed by self-organizing maps (SOM). From these results, we selected the stage-specific genes : one genes (SOM c21 : nadh dehydrogenase subunit 5 gene) for gestational 13.5day, 13 genes (c0 : sialophorin ; spn gene, etc) for postnatal 3 week. The above genes are proposed to be a potential use of biomarker for dioxin exposure in the case of immume dysfunctions.

Keyword

Dioxin; Hematopoietic stem cells; DNA microarray; Gene expression

MeSH Terms

Animals
Antigens, CD43
Bone Marrow
Gene Expression
Hematopoietic Stem Cells*
Mice*
NADH Dehydrogenase
Oligonucleotide Array Sequence Analysis
Oxidoreductases
Stem Cells
Biomarkers
Antigens, CD43
NADH Dehydrogenase
Oxidoreductases
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