Int J Oral Biol.  2014 Dec;39(4):193-199. 10.11620/IJOB.2014.39.4.193.

NaF-induced Autophagy on SCC25 Human Tongue Squamous Cell Carcinoma Cells

• Kang JM
• Lee BY
• Kim IR
• Kim YH
• Yu SB
• Park HR
• Park BS

• KMID: 2170787
• DOI: http://doi.org/10.11620/IJOB.2014.39.4.193

Abstract

Fluoride has been accepted as an important material for oral health and is widely used to prevent dental caries in dentistry. However, its safety is still questioned by some. Autophagy has been implicated in cancer cell survival and death, and may play an important role in oral cancer. This study was undertaken to examine whether sodium fluoride (NaF) modulates autophagy in SCC25 human tongue squamous cell carcinoma cells. NaF demonstrated anticancer activity via autophagic and apoptotic cell death. Autophagic vacuoles were detectable using observed to form by monodansylcadaverine (MDC) and acridine orange (AO). Analysis of NaF-treated SCC25 cells for the presence of biochemical markers revealed direct effects on the conversion of LC-3II, degradation of p62/SQSTM1, cleavage formation of ATG5 and Beclin-1, and caspase activation. NaF-induced cell death was suppressed by the autophagy inhibitor 3-methyladenine (3-MA). NaF-induced autophagy was confirmed as a pro-death signal in SCC25 cells. These results implicate NaF as a novel anticancer compound for oral cancer therapy.

Keyword

NaF; autophagy; apoptosis; human squamous cell carcinoma

MeSH Terms

Acridine Orange
Apoptosis
Autophagy*
Biomarkers
Carcinoma, Squamous Cell*
Cell Death
Cell Survival
Dental Caries
Dentistry
Fluorides
Humans
Mouth Neoplasms
Oral Health
Sodium Fluoride
Tongue*
Vacuoles
Acridine Orange
Fluorides
Sodium Fluoride