Int J Oral Biol.  2011 Sep;36(3):109-116.

Periodontopathogen LPSs Regulate MicroRNA Expression in Human Gingival Epithelial Cells

Affiliations
  • 1Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan 626-870, Korea. jchung@pusan.ac.kr
  • 2Departmet of Oral Medicine, School of Dentistry, Pusan National University, Yangsan 626-870, Korea.
  • 3Department of Oral Pathology, School of Dentistry, Pusan National University, Yangsan 626-870, Korea.

Abstract

Periodontitis results from the activation of host immune and inflammatory defense responses to subgingival plaque bacteria, most of which are gram-negative rods with lipopolysaccharides (LPSs) in their cell walls. LPSs have been known to induce proinflammatory responses and recently it was reported also that they induce the expression of microRNAs (miRNAs) in host cells. In our current study therefore, we aimed to examine and compare the miRNA expression patterns induced by the LPSs of major periodontopathogens in the human gingival epithelial cell line, Ca9-22. The cells were treated with 1 microg/ml of E. coli (Ec) LPS or 5 microg/ml of an LPS preparations from four periodontopathogens Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) for 24 h. After small RNA extraction from the treated cells, miRNA microarray analysis was carried out and characteristic expression profiles were observed. Fn LPS most actively induced miRNAs related to inflammation, followed by Aa LPS, Pi LPS, and Ec LPS. In contrast, Pg LPS only weakly activated miRNAs related to inflammation. Among the miRNAs induced by each LPS, miR-875-3p, miR-449b, and miR-520d-3p were found to be commonly up-regulated by all five LPS preparations, although at different levels. When we further compared the miRNA expression patterns induced by each LPS, Ec LPS and Pi LPS were the most similar although Fn LPS and Aa LPS also induced a similar miRNA expression pattern. In contrast, the miRNA profile induced by Pg LPS was quite distinctive compared with the other bacteria. In conclusion, miR-875-3p, miR-449b, and miR-520d-3p miRNAs are potential targets for the diagnosis and treatment of periodontal inflammation induced by subgingival plaque biofilms. Furthermore, the observations in our current study provide new insights into the inflammatory miRNA response to periodontitis.

Keyword

miRNA; microarray; periodontitis; lipopolysaccharide

MeSH Terms

Bacteria
Biofilms
Cell Wall
Epithelial Cells
Fusobacterium nucleatum
Humans
Inflammation
Lipopolysaccharides
Microarray Analysis
MicroRNAs
Periodontitis
Porphyromonas gingivalis
Prevotella intermedia
RNA
Lipopolysaccharides
MicroRNAs
RNA
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