Biomol Ther.  2013 Jul;21(4):277-283.

Inhibitory Effect of an Urotensin II Receptor Antagonist on Proinflammatory Activation Induced by Urotensin II in Human Vascular Endothelial Cells

• Park SL
• Lee BK
• Kim YA
• Lee BH
• Jung YS

Abstract

In this study, we investigated the effects of a selective urotensin II (UII) receptor antagonist, SB-657510, on the inflammatory response induced by UII in human umbilical vein endothelial cells (EA.hy926) and human monocytes (U937). UII induced inflammatory activation of endothelial cells through expression of proinflammatory cytokines (IL-1beta and IL-6), adhesion molecules (VCAM-1), and tissue factor (TF), which facilitates the adhesion of monocytes to EA.hy926 cells. Treatment with SB-657510 significantly inhibited UII-induced expression of IL-1beta, IL-6, and VCAM-1 in EA.hy926 cells. Further, SB-657510 dramatically blocked the UII-induced increase in adhesion between U937 and EA.hy926 cells. In addition, SB-657510 remarkably reduced UII-induced expression of TF in EA.hy926 cells. Taken together, our results demonstrate that the UII antagonist SB-657510 decreases the progression of inflammation induced by UII in endothelial cells.

Keyword

Urotensin II; Urotensin II receptor antagonist; Adhesion molecules; Cytokines; Tissue factor; Endothelial cells

MeSH Terms

Cytokines
Endothelial Cells*
Human Umbilical Vein Endothelial Cells
Humans*
Inflammation
Interleukin-6
Monocytes
Thromboplastin
Vascular Cell Adhesion Molecule-1
Cytokines
Interleukin-6
Thromboplastin
Vascular Cell Adhesion Molecule-1