J Bone Metab.  2015 Aug;22(3):93-97. 10.11005/jbm.2015.22.3.93.

Comparison of the Chemical Composition of Subchondral Trabecular Bone of Medial Femoral Condyle between with Advanced Osteoarthritis and without Osteoarthritis

Affiliations
  • 1Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea. yougunwon@gmail.com
  • 2Medical Device Development Center, Medical Innovation Foundation, Osong, Korea.
  • 3Department of Orthopaedics, Graduate School of Medicine, Yonsei University, Seoul, Korea.
  • 4Medicine Major, Konyang University College of Medicine, Daejeon, Korea.

Abstract

BACKGROUND
The purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur.
METHODS
Twenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs.
RESULTS
The maximum intensity of the phosphate peak was 2,376.51+/-954.6 for the non-OA group and 1,936.3+/-831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17+/-253.42 for the nonOA group and 261.91+/-205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens.
CONCLUSIONS
The result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.

Keyword

Cartilage; Femur; Osteoarthritis; Spectrum analysis raman

MeSH Terms

Cartilage
Collagen
Femur
Humans
Osteoarthritis*
Spectrum Analysis, Raman
Collagen

Figure

  • Fig. 1 Bone specimen was obtained from medial femoral condyle using a 10 mm diameter trephine.

  • Fig. 2 The Raman spectrum of trabecular bone from the medial femoral condyle with advanced osteoarthritis (OA) (red) and without OA (blue).


Cited by  1 articles

Regional Distribution of Stress on the Distal Femur in Advanced Osteoarthritis
Kwang Kyoun Kim
J Bone Metab. 2018;25(3):175-180.    doi: 10.11005/jbm.2018.25.3.175.


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