J Bacteriol Virol.  2014 Jun;44(2):162-169. 10.4167/jbv.2014.44.2.162.

Microbial Profile of the Stomach: Comparison between Normal Mucosa and Cancer Tissue in the Same Patient

• Seo I
• Jha BK
• Suh SI
• Suh MH
• Baek WK

• KMID: 2168691
• DOI: http://doi.org/10.4167/jbv.2014.44.2.162

Abstract

Gastric cancer is the third most common cancer and the third most frequent cause of cancer mortality in Asia. It is predicted that gastric cancer will remain an important cause of death at least during the next half century because of the increasing number of new cases in an aging population. However, little has been revealed about the role of gastric microbes and their reaction to gastric cancer. In this study, we identified differences in the microbial communities between gastric cancer and normal gastric mucosa by comparing the microbiomes of tissues from the same patients. The clustering analysis results showed different bacterial communities between normal gastric mucosa and gastric cancer. A comparison of bacterial communities at the species level revealed that Helicobacter pylori was significantly reduced in cancer tissue compared to that in normal gastric mucosa in the same patient. A comparison at the genus level showed that Propionibacterium spp., Staphylococcus spp., and Corynebacterium spp. had significantly reduced populations in cancer tissue, whereas Clostridium spp. and Prevotella spp. had significantly increased populations in cancer tissue.

Keyword

Gastric cancer; Helicobacter pylori; Microbiome

MeSH Terms

Aging
Asia
Cause of Death
Clostridium
Corynebacterium
Gastric Mucosa
Helicobacter pylori
Humans
Microbiota
Mortality
Mucous Membrane*
Prevotella
Propionibacterium
Staphylococcus
Stomach Neoplasms
Stomach*

Figure

• 

  Figure 1. Clustering of the samples based on bacterial composition at the species level. Bacterial composition is different between normal gastric mucosa and gastric cancer. Y-axis represents the Bray-Curtis dissimilarity. (A) Whole bacterial species were used for analysis including Helicobacter pylori. (B) Clustering analysis result after excluding H. pylori.

• 

  Figure 2. Relative abundances of Helicobacter pylori in normal gastric mucosa and that of gastric cancer. Normal gastric mucosa has a higher abundance of Helicobacter pylori than that of gastric cancer. Y-axis represents the relative proportion of H. pylori to all bacterial species from each sample. ‘N’ denotes normal gastric mucosa, ‘T’ denotes gastric cancer. Numbers are identification codes for each sample; the same identification code means they were obtained from the same patient.

• 

  Figure 3. More abundant bacterial genera in normal gastric mucosa than that of gastric cancer. Presence of (A) Propionibacterium spp., (B) Staphylococcus spp., and (C) Corynebacterium spp. in normal gastric mucosa and that of gastric cancer. Y-axis represents the relative proportion of each bacterial genus to all bacterial genera from each sample. ‘N’ denotes normal gastric mucosa, ‘T’ denotes gastric cancer. Numbers are identification codes for each sample; the same identification code means they were obtained from the same patient.

• 

  Figure 4. More abundant bacterial genera in gastric cancer than those in normal gastric mucosa. Presence of (A) Clostridium spp. and (B) Prevotella spp. in normal gastric mucosa and that of gastric cancer. Y-axis represents the relative proportion of each bacterial genus to all bacterial genera from each sample. ‘N’ denotes normal gastric mucosa, ‘T’ denotes gastric cancer. Numbers are identification codes for each sample; the same identification code means they were obtained from the same person.

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