Cancer Res Treat.  2001 Jun;33(3):229-235.

Expression of Cyclin D1 and CDK4 in DMBA-Induced Rat Ovarian Cancer

• Kim KK

Abstract

PURPOSE: Ovarian cancer is a common gynecologic malignancy and the leading cause of death in women. It is typically not diagnosed until it has reached the advanced stages. We performed this study to investigate the roles of the proteins related to the G1 cell cycle in ovarian carcinogenesis.
MATERIALS AND METHODS
Immunohistochemistry and Western blot were used to analyse the expression of cyclin Dl and CDK4 in 7, 12-dimethylbenzanthracene- induced ovarian cancer in rats.
RESULTS
The Cyclin D1 and CDK4 labelling index was significantly higher in the ovarian cancers than in the normal ovarian surface epithelium of rats. There was no difference among the cancer types. In Western blot analyses, the expression of cyclin Dl and CDK4 in the ovarian cancers was higher than that in the normal ovarian surface epithelium. A positive correlation was observed between the expressions of the CDK4 and Cyclin D1.
CONCLUSION
The upregulation of cyclin Dl and CDK4 that occurs in DMBA-induced rat ovarian carcinogenesis is likely to be associated with tumor progression. Further studies are needed to investigate the role and function of cyclin Dl and CDK4 in human ovarian cancer.

Keyword

Ovary neoplasm; 7,12-dimethylbenzanthracene; Cyclin Dl; Cyclin-dependent kinase 4

MeSH Terms

9,10-Dimethyl-1,2-benzanthracene
Animals
Blotting, Western
Carcinogenesis
Cause of Death
Cell Cycle
Cyclin D1*
Cyclin-Dependent Kinase 4
Cyclins*
Epithelium
Female
Humans
Immunohistochemistry
Ovarian Neoplasms*
Rats*
Up-Regulation
9,10-Dimethyl-1,2-benzanthracene
Cyclin D1
Cyclin-Dependent Kinase 4
Cyclins