Exp Neurobiol.  2016 Feb;25(1):40-47. 10.5607/en.2016.25.1.40.

Blood Transcriptome Profiling in Myasthenia Gravis Patients to Assess Disease Activity: A Pilot RNA-seq Study

Affiliations
  • 1Department of Neurology, Gyeongsang National University Hospital, Jinju 52727, Korea.
  • 2Department of Bioinformatics, Macrogen Inc., Seoul 08511, Korea.
  • 3Department of Biomedical Science, Hallym University, Chuncheon 24252, Korea.
  • 4Department of Neurology, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul 07061, Korea. nrhong@gmail.com

Abstract

Myasthenia gravis (MG) is an antibody-mediated autoimmune disease characterized by exertional weakness. There is no biomarker to reflect disease activity and guide treatment decision. Here, we reported a pilot blood transcriptome study using RNA sequencing (RNA-seq) that identified differences of 5 samples in active status and 5 in remission from 8 different patients and 2 patients provided samples for both active and remission phase. We found a total of 28 differentially expressed genes (DEGs) possibly related to disease activity (23 up-regulated and 5 down-regulated). The DEGs were enriched for the cell motion and cell migration processes in which included were ICAM1, CCL3, S100P and GAB2. The apoptosis and cell death pathway was also significantly enriched, which includes NFKBIA, ZC3H12A, TNFAIP3, and PPP1R15A. Our result suggests that transcript abundance profiles of the genes involved in cell trafficking and apoptosis may be a molecular signature of the disease activity in MG patients.

Keyword

Transcriptome; RNA sequencing; myasthenia gravis; cell migration; apoptosis

MeSH Terms

Apoptosis
Autoimmune Diseases
Cell Death
Cell Movement
Gene Expression Profiling*
Humans
Myasthenia Gravis*
Sequence Analysis, RNA
Transcriptome*
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