Exp Neurobiol.  2009 Jun;18(1):37-47. 10.5607/en.2009.18.1.37.

Differentiation of Rat Neural Stem Cells Following Transplantation in the Brain of Huntington's Disease Rat Model

• Ryu HL
• Lee SY
• Park K
• Kim C
• Jin BK
• Min CK

• KMID: 2165295
• DOI: http://doi.org/10.5607/en.2009.18.1.37

Abstract

Stem cells provide an important means for regenerative medicine due to the capacity to generate multiple types of differentiated cells and at the same time to maintain self-renewal. To identify the therapeutic effect of the transplantation of neural stem cells, differentiation and migration capacity of the neural stem cells that were isolated from E14 rat embryo and maintained in culture were examined after transplantation to the striatum of the quinolinic acid (QA)-induced Huntington's disease rat model. in vitro co-culture of the neural stem cells with the mixture of primary neurons and astrocytes promoted the maturation and the synapse formation of neuronal progenies of neural stem cells. Following the implantation, the neural stem cells survived, differentiated, and migrated in the damaged striatum region, exhibiting immunoreactivities against nestin, Tuj-1, GFAP, GAD(67) and synapsin 1 to a varying degree. These data provide clear evidence supporting that the neural stem cells isolated from the rat embryo and maintained in the primary culture have a multiple capacity to differentiate into neurons or glial cells both in vitro and in vivo.

Keyword

neural stem/progenitor cells; Huntington's disease; striatal graft; quinolinic acid

MeSH Terms

Animals
Astrocytes
Brain
Coculture Techniques
Embryonic Structures
Huntington Disease
Intermediate Filament Proteins
Nerve Tissue Proteins
Neural Stem Cells
Neuroglia
Neurons
Quinolinic Acid
Rats
Regenerative Medicine
Synapses
Transplants
Intermediate Filament Proteins
Nerve Tissue Proteins
Quinolinic Acid