Ann Dermatol.  2000 Dec;12(4):243-246. 10.5021/ad.2000.12.4.243.

Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line

• Park KC
• Lim JG
• Kim DS
• Choi HO
• Kim KH
• Cho KH
• Youn JI

• KMID: 2163992
• DOI: http://doi.org/10.5021/ad.2000.12.4.243

Abstract

BACKGROUND
Ultraviolet B (UVB) irradiation can induce apoptosis of melanocytes and melanoma cells. However, mechanism of UVB-induced apoptosis of melanoma cells is not clarified yet.
OBJECTIVE
Our purpose was to study the molecular mechanism of UVB-induced apoptosis of melanoma cells.
METHODS
G361 lightly pigmented melanoma cells were analyzed for apoptotic mechanism by flow cytometry and western blotting.
RESULTS
G361 melanoma cells showed apoptotic features with gradual increment of UVB doses by MTT and flow cytometry. Western blotting disclosed activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) after UVB irradiation.
CONCLUSION
In this study, we showed that UVB-induced apoptosis of melanoma cells is mediated by PARP activation which is induced by caspase cascade.

Keyword

Melanoma cells; Ultraviolet B; Apoptosis; Caspase

MeSH Terms

Apoptosis*
Blotting, Western
Caspase 3
Caspases*
Cell Line*
Flow Cytometry
Humans*
Melanocytes
Melanoma*
Caspase 3
Caspases