Immune Netw.  2008 Sep;8(3):98-105. 10.4110/in.2008.8.3.98.

Screening of Anti-Atopic Dermatitis Material by Using NC/Nga Mouse Whole Blood System

• Park D
• Kim YU

• KMID: 2163199
• DOI: http://doi.org/10.4110/in.2008.8.3.98

Abstract

BACKGROUND: Allergic inflammation was induced by activated Th2 lymphocytes, leading to IgE production and eosinophil activation. A Th2 disproportion was shown in atopic children soon after birth. During specific allergen stimulation, an increase of Th2 cells was observed in most cases. In this study, we prepared new screening "whole blood" system for searching the anti-atopic materials. Cytokine production and IgE secretion from whole blood system were assessed and we confirmed the results by using animal system.
METHODS
Pathological features in NC/Nga mice are similar to those observed in human atopic dermatitis. Whole blood from NC/Nga mouse was stimulated by using TNCB (Th2 activator) or candidate materials of anti-atopic dermatitis, and the production of cytokines (IL-4, IL-12, and IFN-gamma) were measured by ELISA. In order to confirm the results of whole blood system, in vivo test was done by using NC/Nga mice.
RESULTS
In whole blood system, LPS and extracts of green tea, hardy orange and onion induced the production of IL-12 and IFN-gamma while they reduced the production of IL-4. Also, LPS and extracts of onion reduced IgE production. Though atopic dermatitis was observed from a mouse stimulated with TNCB, it was not when a mouse was co-stimulated in LPS or extracts of onion. The results are same as those observed in whole blood system.
CONCLUSION
Whole blood system was simple and speedy methods for searching a materials compared with the conventional high-cost animal system. And the results using whole blood system was proved to be reliable in our experiments for screening anti-atopic material. We expect that the system can be applied to other experiments for searching similar materials.

Keyword

helper T cell; Th1; Th2; atopic dermatitis; asthma; IL-4; IL-12; IFN-gamma

MeSH Terms

Animals
Asthma
Child
Citrus sinensis
Cytokines
Dermatitis
Dermatitis, Atopic
Enzyme-Linked Immunosorbent Assay
Eosinophils
Humans
Immunoglobulin E
Inflammation
Interleukin-12
Interleukin-4
Lymphocytes
Mass Screening
Mice
Onions
Parturition
Tea
Th2 Cells
Cytokines
Immunoglobulin E
Interleukin-12
Interleukin-4
Tea