Yonsei Med J.  2008 Oct;49(5):804-810. 10.3349/ymj.2008.49.5.804.

The Expression of Adiponectin Receptors and the Effects of Adiponectin and Leptin on Airway Smooth Muscle Cells

• Shin JH
• Kim JH
• Lee WY
• Shim JY

• KMID: 2158199
• DOI: http://doi.org/10.3349/ymj.2008.49.5.804

Abstract

PURPOSE
Obesity is a major risk factor for asthma and it influences airway smooth muscle function and responsiveness. Adiponectin is inversely associated with obesity and its action is mediated through at least 2 cell membrane receptors (AdipoR1 and AdipoR2). Leptin is positively associated with obesity. We investigated whether human airway smooth muscle (ASM) cells express adiponectin receptors and whether adiponectin and leptin regulate human ASM cell proliferation and vascular endothelial growth factor (VEGF) release. MATERIALS AND METHODS: Human ASM cells were growth-arrested in serum-deprived medium for 48 hours and then stimulated with PDGF, adiponectin and leptin. After 48 hours of stimulation, proliferation was determined using a cell proliferation ELISA kit. Human AdipoR1 and -R2 mRNA expressions were determined by RT-PCR using human-specific AdipoR1 and -R2 primers. Concentrations of VEGF, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha in cell culture supernatant were determined by ELISA. RESULTS: Both AdipoR1 and AdipoR2 mRNA were expressed in the cultured human ASM cells. However, adiponectin did not suppress PDGF-enhanced ASM cell proliferation, nor did leptin promote ASM cell proliferation. Leptin promoted VEGF release by human ASM cells, while adiponectin did not influence VEGF release. Neither leptin nor adiponectin influenced MCP-1 secretion from human ASM cells. Adiponectin and MIP-1alpha were not secreted by human ASM cells. CONCLUSION: Human ASM cells expressed adiponectin receptors. However, adiponectin did not regulate human ASM cell proliferation or VEGF release, while leptin stimulated VEGF release by human ASM cells.

Keyword

Smooth muscle cells; cell proliferation; vascular endothelial growth factor; leptin; adiponectin; receptors

MeSH Terms

Adiponectin/metabolism/*pharmacology/physiology
Cell Proliferation/*drug effects
Cells, Cultured
Chemokine CCL2/metabolism
Chemokine CCL3/metabolism
Humans
Leptin/metabolism/*pharmacology/physiology
Myocytes, Smooth Muscle/cytology/drug effects/*metabolism
Obesity/metabolism
Platelet-Derived Growth Factor/metabolism
Receptors, Adiponectin/*metabolism
Respiratory System/cytology/metabolism
Vascular Endothelial Growth Factor A/metabolism