J Korean Med Sci.  2013 Jan;28(1):62-66. 10.3346/jkms.2013.28.1.62.

Antimicrobial Susceptibility of Stenotrophomonas maltophilia Isolates from Korea, and the Activity of Antimicrobial Combinations against the Isolates

Affiliations
  • 1Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, Seoul, Korea.
  • 2Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea. hlseo@cha.ac.kr
  • 3Department of Laboratory Medicine, Jeju National University School of Medicine, Jeju, Korea.
  • 4Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, Korea.
  • 5Department of Laboratory Medicine, Inje University College of Medicine, Seoul, Korea.
  • 6Department of Laboratory Medicine, Sooncheonhyang University College of Medicine, Gumi, Korea.
  • 7Department of Laboratory Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.
  • 8Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
  • 9Department of Laboratory Medicine, Pusan National University School of Medicine, Busan, Korea.
  • 10Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
  • 11Department of Laboratory Medicine, Chonbuk National University Medical School, Jeonju, Korea.

Abstract

The aim of this study was to determine antimicrobial susceptibility of recent clinical Stenotrophomonas maltophilia isolates from Korea, and to compare the activity levels of several combinations of antimicrobials. A total of 206 non-duplicate clinical isolates of S. maltophilia was collected in 2010 from 11 university hospitals. Antimicrobial susceptibility testing was performed using the Clinical Laboratory Standards Institute agar dilution method. In vitro activity of antimicrobial combinations was tested using the checkerboard method. The susceptibility rates to trimethoprim-sulfamethoxazole and minocycline were 96% and 99%, respectively. The susceptibility rate to levofloxacin was 64%. All of four antimicrobial combinations showed synergy against many S. maltophilia isolates. A combination of trimethoprim-sulfamethoxazole plus ticarcillin-clavulanate was most synergistic among the combinations. None of the combinations showed antagonistic activity. Therefore, some of the combinations may be more useful than individual drugs in the treatment of S. maltophilia infection. Further clinical studies are warranted to validate our in vitro test results.

Keyword

Stenotrophomonas maltophilia; Combination Drug Therapy; Trimethoprim-Sulfamethoxazole; Ticarcillin-Clavulanate

MeSH Terms

Anti-Infective Agents/*pharmacology
Gram-Negative Bacterial Infections/microbiology
Hospitals, University
Humans
Microbial Sensitivity Tests
Minocycline/pharmacology
Ofloxacin/pharmacology
Republic of Korea
Stenotrophomonas maltophilia/*drug effects/isolation & purification
Trimethoprim-Sulfamethoxazole Combination/pharmacology
Anti-Infective Agents
Minocycline
Trimethoprim-Sulfamethoxazole Combination
Ofloxacin

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