J Korean Med Sci.  2005 Dec;20(6):1017-1022. 10.3346/jkms.2005.20.6.1017.

Cytokine Production by Whole Blood Cells: Relationship to Interleukin Gene Polymorphism and Bone Mass

• Kim JG
• Ku SY
• Lim KS
• Jee BC
• Suh CS
• Kim SH
• Choi YM
• Moon SY

• KMID: 2157755
• DOI: http://doi.org/10.3346/jkms.2005.20.6.1017

Abstract

The aims of this study were to investigate the relationships between the production of interleukin-1 (IL-1), and IL-6 system by whole blood cells, and bone mineral density (BMD), and polymorphisms in IL-1 system and IL-6 gene in postmenopausal Korean women. The production of IL-1alpha, IL-1beta, IL-1 receptor antagonist (IL-1ra), IL-6, and soluble IL-6 receptor (sIL-6r) by lipopolysaccharide-stimulated whole blood cells was measured by ELISA in 110 subjects. Serum osteocalcin, C-telopeptide of type I collagen, and BMD at lumbar spine and proximal femur were measured. IL-1alphaC(-889)T polymorphism, IL-1beta C(-511)T polymorphism, 86-base pair variable number tandem repeat polymorphism in the IL-1ra gene, and IL-6 C(-634)G polymorphism were analyzed. The production of IL-1beta correlated positively with BMD at femoral neck, whereas the production of other ILs did not correlate with BMD at the skeletal sites examined. No significant differences in the production of ILs were observed among normal, osteopenic and osteoporotic postmenopausal women, and among the different IL system polymorphisms groups studied. No correlation between bone turnover markers and the production of ILs was noted. In conclusion IL-1beta may regulate bone metabolism at femoral neck, and the IL system polymorphism do not affect the production of ILs by whole blood cells.

Keyword

Interleukins; Polymorphism, Genetic; Bone Demineralization, Pathologic; Blood Cells

MeSH Terms

Aged
Blood Cells/drug effects/immunology
Bone Density/*genetics/*immunology
Bone Diseases, Metabolic/blood/genetics/immunology
Cytokines/*biosynthesis/blood
Female
Humans
In Vitro
Interleukin-1/biosynthesis/blood/genetics
Interleukin-6/biosynthesis/blood/genetics
Interleukins/*genetics
Lipopolysaccharides/pharmacology
Middle Aged
Osteoporosis, Postmenopausal/blood/genetics/immunology
*Polymorphism, Genetic
Receptors, Interleukin-6/biosynthesis/blood/genetics
Research Support, Non-U.S. Gov't
Sialoglycoproteins/biosynthesis/blood/genetics

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