J Korean Soc Microbiol.  1998 Aug;33(4):415-423.

Mutational Analysis of p53, p21WAF1 and p16INK4a in Human Cervical Carcinomas

• Suh SI
• Choi EJ
• Baek WK
• Suh MH
• Cho CH
• Lee TS
• Cha SD

Abstract

Human papillomavirus (HPV) infection has been implicated to be an important causative factor for the development of cervical carcinoma. p53 gene mutation is common in human malignancies, and it is suggested that p53 function is inactivated either by complex formation with HPV E6 product or by gene mutation in cervical carcinoma. Forty-six cervical carcinoma samples were evaluated for the presence of mutations in p53, p21WAF1 and p16INK4a genes with polymerase chain reaction (PCR), single stranded conformational polymorphism (SSCP) analysis and DNA sequencing. The status of HPV infection in tumor tissues was analysed by PCR. Forty-two of 46 cervical carcinomas showed HPV infection. In four HPV-negative cervical carcinomas there was no abnormal mobility-shifted band in PCR-SSCP analysis of p53, p21WAF1 and p16INK4a. However, two out of 42 HPV infected cervical carcinomas showed abnormal mobility shifted band in p16INK4a exon 3, and subsequent analysis of them revealed that mutations were not in coding region but in 3' untranslated region (UTR) of axon 3. These results suggest that HPV-negative carcinoma may arise via a pathway independent of p53, p21WAF1 and p16INK4a mutational inactivation. But it remains to be determined whether disruption of the 3' UTR of p16INK4a mRNA leads to an increased risk for cervical carcinomas.

Keyword

Cervical carcinoma; p53; p21WAF1; p16INK4a; Mutation analysis; HPV

MeSH Terms

3' Untranslated Regions
Axons
Clinical Coding
Exons
Genes, p16
Genes, p53
Humans*
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
RNA, Messenger
Sequence Analysis, DNA
3' Untranslated Regions
RNA, Messenger