J Korean Soc Microbiol.  2000 Apr;35(2):149-157.

Up-regulation of prothymosin alpha in THP-1 cells infected with Mycobacterium tuberculosis

• Song HY
• Jang KS
• Byoun HS
• Lee SJ
• Kim JK
• Choe YK
• Ko KK

Abstract

Mycobacterium tuberculosis is capable of growing and survival within macrophage. The purpose of this study was to identify the genes regulated by infection of mycobacteria in human monocytic THP-1 cells. We used the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) and nothern blot analysis to confirm the differentially expressed genes from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv, heat-kille Mycobacterium tuberculosis H37Rv and live Mycobacterium bovis BCG. Among many up or down-regulated clones, 27 clones were sequenced and compared with known genes on GenBank. Thirteen of over-expressed clones from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv were identical to human prothymosin alpha, eight were novel clones and six clones showed homology with Human ferritin H chain, Escherichia coli bgl, Mouse RNA-dependent EIF-2 alpha kinase, E. coli htrL, Hyaluronan receptor and T cell receptor. Our result suggests that Mycobacterium tuberculosis might regulate prothymosin alpha gene transcription in monocytic THP-1 cell.

MeSH Terms

Animals
Antigens, CD44
Clone Cells
Databases, Nucleic Acid
Escherichia coli
Eukaryotic Initiation Factor-2
Ferritins
Humans
Macrophages
Mice
Mycobacterium bovis
Mycobacterium tuberculosis*
Mycobacterium*
Phosphotransferases
Receptors, Antigen, T-Cell
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation*
Antigens, CD44
Eukaryotic Initiation Factor-2
Ferritins
Phosphotransferases
Receptors, Antigen, T-Cell