J Korean Surg Soc.
2000 Sep;59(3):391-396.
Reduced Messenger RNA Expression of the Neuronal Nitric Oxide Synthase Gene in Infantile Hypertrophic Pyloric Stenosis
- Affiliations
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- 1Department of Surgery, College of Medicine, Inha University, Inchon, Korea.
- 2Department of Pharmacology, College of Medicine, Inha University, Inchon, Korea.
Abstract
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PURPOSE: Nitric oxide synthesized by neuronal nitric oxide synthase (nNOS) has been described as
a mediator of smooth muscle relaxation in the mammalian gastrointestinal tract. Impaired expression of
the nNOS gene is suggested in the development of infantile hypertrophic pyloric stenosis (IHPS). We
examined the expression of nNOS mRNA in pyloric muscle biopsy specimens obtained from 8 patients
with IHPS and attempted to correlate the results with the clinical characteristics. METHODS: The expression
of nNOS mRNA in pyloric muscle biopsy specimens for 8 patients with IHPS was examined using a
reverse transcription- polymerase chain reaction (RT-PCR) technique. For the control, a smooth muscle
layer specimen of a neonate with a normal pylorus was used. RESULTS: In the control specimen, the level
of nNOS mRNA expression was 48.4% of beta-actin mRNA. In the two thinnest (each 3 mm) of pyloric
muscle thicknesses as determined by ultra-sonography, the expressed nNOS mRNA were 16.7% and
30.3%. The two thickest (each 8.3 mm) expressed as 35.3% and 22.9% nNOS. The two samples from
the earliest age of symptomatic onset (1 day, 7 days after birth) expressed as 25.6% and 4.8%. The
two from the latest age of onset (each 30 days) expressed as 7.4% and 10.5%. The control specimen
revealed a higher level of nNOS mRNA expression than those of the IHPS specimens. There was no
significant correlation between the clinical characteristics and the levels of nNOS mRNA in the IHPS
specimens. CONCLUSION: Since a low level of nNOS mRNA expression may lead to impaired production
of NO, our observations indicate that the hypertrophic pyloric muscle of an IHPS patient may be the
result of a reduced expression of the nNOS gene at the mRNA level. In IHPS patients, there was no
correlation between the clinical characteristics and the levels of expressed nNOS mRNA.