J Clin Neurol.  2007 Sep;3(3):127-132. 10.3988/jcn.2007.3.3.127.

Screening of the A11084G Polymorphism and Scanning of a Mitochondrial Genome SNP in Korean Migraineurs

• Kang L
• Lee ST
• Im W
• Kim SC
• Hun KS
• Kim BK
• Kim M

• KMID: 2135490
• DOI: http://doi.org/10.3988/jcn.2007.3.3.127

Abstract

BACKGROUND AND PURPOSE
Migraine is a genetically heterogeneous disorder that is frequently associated with a familial history, and mitochondrial dysfunction has been suggested to be associated with its pathogenesis. We screened and scanned mitochondrial gene polymorphisms to determine the significance of mitochondrial DNA mutations in Korean migraineurs.
METHODS
One hundred and sixty-four migraineurs aged 33.9+/-11.7 years (mean+/-SD range 12 to 65 years) were studied. Clinical data of the familial history were obtained, and blood samples were collected for DNA purification. An A-to-G substitution at mitochondrial DNA (mtDNA) position 11,084 (A11084G) was determined by a polymerase chain reaction (PCR) with BsmI restriction. In addition, new single-nucleotide polymorphism (SNP) sites in the mitochondrial genome were scanned for using PCR and direct sequencing.
RESULTS
Ninety-eight migraine patients (59.8%) had a maternal familial history. The A11084G polymorphism, which was previously reported in 25% of Japanese migraineurs, was not evident in our Korean migraine patients. However, scanning of new SNP sites in mtDNA revealed six candidate SNPs whose incidences were higher in migraine patients than in normal subjects.
CONCLUSIONS
Our study found no association between the A11084G polymorphism in mitochondrial DNA and migraine in Koreans. However, we found potential new mitochondrial SNP sites in Korean migraineurs, which warrant further investigation.

Keyword

Migraine; Mitochondria; Single-Nucleotide Polymorphism

MeSH Terms

Asian Continental Ancestry Group
DNA
DNA, Mitochondrial
Genes, Mitochondrial
Genome, Mitochondrial*
Humans
Incidence
Mass Screening*
Migraine Disorders
Mitochondria
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
DNA
DNA, Mitochondrial

Figure

• 

  Figure 1 A11084G polymorphism of mtDNA. PCR products digested with BsmI were sized on a 1% TAE agarose gel with a 100-bp DNA ladder marker (lane 1). Lane 2 is an amplified 402-bp mtDNA encompassing position 11,084. Lane 4 is a 600-bp positive control containing the BsmI site at position 13,309-13,314. Lanes 3 and 5 are PCR products digested with BsmI from lanes 2 and 4, respectively. The patterns of the PCR products from migraineurs and control subjects were identical in lanes 2 and 3.

• 

  Figure 2 Representative sequencing results of each mtDNA SNP that is possibly related to migraine. Arrows indicate the SNP sites.

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